The microscopic study of bone (self made slide)

[Raul]

Hello all, I wanted to share some images from a new project of mine.

The good donor for this sample is an unknown pig.
I will also post pictures of the procedure for the processing of the bone if anyone is interested.

Hope you enjoy it.








The next is with the iris diaphragm almost fully closed

[billbillt]

These are great!... YES!... I would be very interested in seeing more!...

BillT

[Raul]

Thank you very much billbillt.
As promised the protocol is the following:
The samples (left is a chicken femur and right bone unknown origin from a pig)

First I tried the femur but was only able to section it in half (still trying on it)

For the pig bone I used a scalpel and tweezers to remove a small piece

After I used fine sandpaper to grind and at the end polish the sample

The sample was 0.132 mm thick when I decided to stop because it seemed to fragile to polish more


I mounted the bone in glycerin because of its high RI and the fact that glycerin is used as a clearing agent for bone (making the sample more transparent)

The sample showed great details using different filters and didn't require staining

[billbillt]

Hi Raul,
These are very good!... A very interesting project... Thanks for sharing!....

BillT

[billben74]

Brilliant.
Always good to see people pushing out the sectioning skills.
Lots of work, nice result.

What about a tooth?;)

[Raul]

Thank you billbillt and billben74 i appreciate your feedback very much. billben74 yes a tooth is a very good and interesting idea, that would be a future project as soon as I find a donor for that (hope it won't be me because I have my permanent teeth).