Daphnia

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c-krebs
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Joined: Sat Jun 18, 2016 7:22 pm

Daphnia

#1 Post by c-krebs » Sat Jun 25, 2016 8:18 pm

Image

Image

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75RR
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Location: Estepona, Spain

Re: Daphnia

#2 Post by 75RR » Sat Jun 25, 2016 8:22 pm

Simply beautiful - thanks
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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zzffnn
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Re: Daphnia

#3 Post by zzffnn » Sat Jun 25, 2016 8:31 pm

Your photos always amaze me!
Were those simple darkfield, or DIC with black background?

billbillt
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Re: Daphnia

#4 Post by billbillt » Sat Jun 25, 2016 9:04 pm

WOW!!.. I am simply blown away!!... FANTASTIC!!...

BillT

Rodney
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Re: Daphnia

#5 Post by Rodney » Sat Jun 25, 2016 9:52 pm

That is really a good photo of daphnia, what type of camera did you use to capture the photo?

Rodney

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c-krebs
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Re: Daphnia

#6 Post by c-krebs » Sat Jun 25, 2016 10:32 pm

zzffnn wrote:Were those simple darkfield, or DIC with black background?
Rodney wrote:what type of camera did you use to capture the photo?
The camera was a Canon T3i. The microscope was a "hybrid" Nikon MM-11/Olympus scope I use a great deal. You can see the scope below.

Image

It was simple darkfield (no DIC) but not the typical condenser type. The picture below shows a set-up similar top what was used. The slide was placed over the opening in the stage. Under it there was no condenser. A piece of black flocked material ("Protostar") was placed a few inches underneath the stage opening to provide the black background. A "ring diffuser" (in this case a piece of ping-pong ball) was placed on the slide around the subject and it was illuminated from the sides via LED lights. (I no longer use the Ikea lights as seen in this older picture. I now use some LED lights that I put together and described here. Two of those lights were used from the sides.

Image

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75RR
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Re: Daphnia

#7 Post by 75RR » Sat Jun 25, 2016 11:05 pm

Had seen your hybrid setup before but had assumed it was for taking photos of insects - had no idea it could be used with a wet slide and to such great effect. What would you call that illumination technique?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

JimT
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Re: Daphnia

#8 Post by JimT » Sat Jun 25, 2016 11:14 pm

The slide was placed over the opening in the stage. Under it there was no condenser. A piece of black flocked material ("Protostar") was placed a few inches underneath the stage opening to provide the black background. A "ring diffuser" (in this case a piece of ping-pong ball) was placed on the slide around the subject and it was illuminated from the sides via LED lights
So, nothing special :D :D

Fantastic images and great process. We want more!!!

JimT

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rnabholz
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Re: Daphnia

#9 Post by rnabholz » Sun Jun 26, 2016 4:35 am

A stunning group of images, and your innovative approach to lighting is really interesting.

The results are certainly worth the effort.

Well done!

Rod

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gekko
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Re: Daphnia

#10 Post by gekko » Sun Jun 26, 2016 4:13 pm

I can think of only two words: perfection and poetry. Thank you for the post and for the explanation.

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KurtM
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Re: Daphnia

#11 Post by KurtM » Mon Jun 27, 2016 3:38 am

Great to see Mr. Krebs here! Knockout imagery as always, and especially love the "behind the scenes" bits - it's nothing if not wonderfully inspirational.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

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vasselle
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Re: Daphnia

#12 Post by vasselle » Tue Jun 28, 2016 2:24 pm

Bonjour
Superbes photos.
Et j'aime énormément votre travail que vous faite.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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