Larger Diatoms Just For Fun

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Larger Diatoms Just For Fun

#1 Post by KurtM » Mon Jul 11, 2016 12:08 am

Just having a little fun with the microscope and camera, mostly fooling around testing a trinocular head I refurbished - finally got a black one to match the rest of my Zeiss WL stand and had to clean and re-lube the innards to make it work like I want. This is a Raymond Hummerlink slide, don't know much about the sample other than that, except to say it's rich in Pinnularia and Nitzschia.

First image is a Pinnularia through the dreaded 40x/.65 lens, DIC - whaddya think, 75? Second is Pinnulria through the 100x/1.25 lens, DIC, 1st order red compensator; not a great image, but I liked the colors! Next two are same through the 100x/1.25, DIC. Finally, a Nitschia that was flat enough to actually do some good with a single frame.

I really, really need to conquer stacking... :P
Attachments
7-10-16 0086_lzn 1024px.jpg
7-10-16 0086_lzn 1024px.jpg (79.28 KiB) Viewed 7464 times
7-10-16 0035_lzn 1024px.jpg
7-10-16 0035_lzn 1024px.jpg (114.56 KiB) Viewed 7464 times
7-10-16 0069_lzn 1024px.jpg
7-10-16 0069_lzn 1024px.jpg (144.12 KiB) Viewed 7464 times
7-10-16 0009_lzn 1024px.jpg
7-10-16 0009_lzn 1024px.jpg (175.36 KiB) Viewed 7464 times
7-10-16 0001_lzn 1024px.jpg
7-10-16 0001_lzn 1024px.jpg (130.93 KiB) Viewed 7464 times
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#2 Post by KurtM » Mon Jul 11, 2016 12:13 am

The "new & improved" Zeiss WL. 8-)
Attachments
new trinoc portrait 1024px.jpg
new trinoc portrait 1024px.jpg (230.14 KiB) Viewed 7462 times
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Larger Diatoms Just For Fun

#3 Post by 75RR » Mon Jul 11, 2016 12:13 am

You seem to have stepped up your game! Well done. Those are a pleasure to look at.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Larger Diatoms Just For Fun

#4 Post by 75RR » Mon Jul 11, 2016 12:15 am

Good call on that new head - looks much nicer.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
rnabholz
Posts: 3086
Joined: Thu Jan 08, 2015 10:11 pm
Location: Iowa USA
Contact:

Re: Larger Diatoms Just For Fun

#5 Post by rnabholz » Mon Jul 11, 2016 12:30 am

I am certainly no DIC expert, but they all look great to me. Outstanding resolution on all.

I would certainly be pleased if they were mine.

The new head is like a crown on royalty. That is one sweet rig.

Congrats on all.

Rod

User avatar
exmarine
Posts: 247
Joined: Mon May 09, 2016 1:16 am
Location: Cambridgeshire UK

Re: Larger Diatoms Just For Fun

#6 Post by exmarine » Mon Jul 11, 2016 12:48 am

Hi Kurt,
You have hit the high spot with those photo's, just brilliant!!!!!!!

The definition on each diatom is outstanding. Really pleased for you that you have found a way to bring pleasure to many diatom enthusiast. Fantastic stuff.
Thank you :shock:
Best regards
exmarine :x

uses Watson 'Service' 1950 compound.
uses Watson Stereo 1960 ish.

User avatar
hb2sbdb
Posts: 31
Joined: Sat Apr 16, 2016 1:47 pm

Re: Larger Diatoms Just For Fun

#7 Post by hb2sbdb » Mon Jul 11, 2016 1:04 am

Wow. Even better looking diatoms now Kurt!

This kind of quality has to come from mastering of both the microscope components (objectives + condenser fiddling :) ) and the way you set up the photographic system.

Sorry if you had explained this somewhere on this site already, but are you using afocal with your setup?

Thanks

User avatar
c-krebs
Posts: 201
Joined: Sat Jun 18, 2016 7:22 pm

Re: Larger Diatoms Just For Fun

#8 Post by c-krebs » Mon Jul 11, 2016 2:08 am

Kurt,

These are really nice. I'll reserve judgement on the 40X (always best to make a judgement of subject that "fills-the-frame" more). The 100X looks really good.

On the second image... which is excellent... you have too much of it's splendor buried in the dark tones. Check out the histogram. A simple levels adjustment brings it to life.

Image

User avatar
McConkey
Posts: 338
Joined: Fri Feb 19, 2016 4:33 am
Location: Alberta, Canada

Re: Larger Diatoms Just For Fun

#9 Post by McConkey » Mon Jul 11, 2016 2:32 am

Oh wow, excellent images Kurt! These are very well done! Really nice looking setup you got going!

What are you using for stacking/how many images were stacked?
Karl
AO21 with Canon M3

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#10 Post by KurtM » Mon Jul 11, 2016 4:03 am

hb2sbdb wrote:...are you using afocal with your setup?
Beats me what it's called. A dSLR (Canon T3i) is mounted to the trinoc photo tube so I'd say no, but there's a 2.5x photographic eyepiece inside the tube so maybe it is? Never mistake me for somebody who knows what he's doing. :?
c-krebs wrote:you have too much of it's splendor buried in the dark tones.
Not the first time I've been fussed for too-dark photos. I like 'em dark. I know I need to knock it off, but I just can't stop. Must have goth-emo tendencies. I'll try to behave better in the future (and learn to read histograms). 8-)
McConkey wrote:What are you using for stacking/how many images were stacked?
No stacks, that's the problem! All the above are single frames. My efforts at stacking with Combine ZP and Picolay have resulted in pure #@&^%. I am considering trying Zerene (aye, blame it on the software!). :roll:

THANKS, all! :mrgreen:

EDIT: c-krebs, I sent you a PM.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
McConkey
Posts: 338
Joined: Fri Feb 19, 2016 4:33 am
Location: Alberta, Canada

Re: Larger Diatoms Just For Fun

#11 Post by McConkey » Mon Jul 11, 2016 4:28 am

For single frames these look really good! With the level of detail i thought they were stacks! Great job!

I've had mixed results with picolay, some great some not so! I havn't heard of Zerene but i'll check it out!
KurtM wrote:Never mistake me for somebody who knows what he's doing. :?
We never do! :P
Karl
AO21 with Canon M3

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#12 Post by KurtM » Mon Jul 11, 2016 2:24 pm

In the PM to c-krebs I invited him to perform a demonstration of post processing on image 2, as many of us are sure to be interested in his opinions on the matter. But as I have had other commentary on it, I hereby extend the invitation to anyone who wishes to play with it. Shoot me an email and I'll send full rez original file of the image.

In the meantime, here's another shot I took at it, and I invite any and all comments/critique.
Attachments
7-10-16 0035_lzn 2 1024px.jpg
7-10-16 0035_lzn 2 1024px.jpg (151.5 KiB) Viewed 7403 times
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#13 Post by KurtM » Mon Jul 11, 2016 2:25 pm

McConkey wrote:We never do! :P
:lol:

Karl, Zerene and Helicon are the two big names in focus stacking software, and cost money. CombineZP and Picolay are, of course, the freebie programs.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

JimT
Posts: 3247
Joined: Fri Oct 24, 2014 1:57 pm

Re: Larger Diatoms Just For Fun

#14 Post by JimT » Mon Jul 11, 2016 3:37 pm

I'd be happy to stick any one of them on my refrigerator :)

billbillt
Posts: 2895
Joined: Mon Nov 10, 2014 10:01 pm

Re: Larger Diatoms Just For Fun

#15 Post by billbillt » Mon Jul 11, 2016 3:38 pm

To my untrained eye, these are great!!.. Very good resolution..

BillT

User avatar
McConkey
Posts: 338
Joined: Fri Feb 19, 2016 4:33 am
Location: Alberta, Canada

Re: Larger Diatoms Just For Fun

#16 Post by McConkey » Mon Jul 11, 2016 4:05 pm

PM sent!

The latest image you posted, again, has a great amount of detail! Excellent job!
Karl
AO21 with Canon M3

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Larger Diatoms Just For Fun

#17 Post by gekko » Mon Jul 11, 2016 6:02 pm

Kurt, to my [bad] eyes, they are all sharp and very beautiful. Thank you for sharing them.

User avatar
McConkey
Posts: 338
Joined: Fri Feb 19, 2016 4:33 am
Location: Alberta, Canada

Re: Larger Diatoms Just For Fun

#18 Post by McConkey » Tue Jul 12, 2016 1:54 am

I took up Kurts invitation and requested a full size image to play with. With his permission i'm posting them.

Please keep in mind this is an alteration based on my personal taste and is in no way a critique on Kurts awesome work. Just a show and tell of how images can be altered in post processing. Diatoms are the same in both images...just one has a lighter background.
Attachments
Kurts Diatom2forum.jpg
Kurts Diatom2forum.jpg (268.63 KiB) Viewed 7347 times
Kurts Diatom1forum.jpg
Kurts Diatom1forum.jpg (354.59 KiB) Viewed 7347 times
Karl
AO21 with Canon M3

User avatar
vasselle
Posts: 2763
Joined: Sat Oct 25, 2014 5:32 pm
Location: France

Re: Larger Diatoms Just For Fun

#19 Post by vasselle » Tue Jul 12, 2016 3:56 pm

Bonjour
Very nice photos
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

User avatar
billben74
Posts: 1020
Joined: Sat Dec 26, 2015 10:33 pm
Location: Cambridge, UK

Re: Larger Diatoms Just For Fun

#20 Post by billben74 » Tue Jul 12, 2016 4:22 pm

Well I think these images are top notch.

I really like the colours on

7-10-16 0035_lzn 2 1024px.jpg

against the dark background.

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Larger Diatoms Just For Fun

#21 Post by gekko » Tue Jul 12, 2016 4:35 pm

Karl, I think your versions are also very beautiful

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#22 Post by KurtM » Tue Jul 12, 2016 4:43 pm

Thanks for kind comments, all!

Pretty cool demonstration of what post processing can do, Karl, now how about a bit of info on what you did, and what you did it with? I'm curious as to how you change the background like that.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
McConkey
Posts: 338
Joined: Fri Feb 19, 2016 4:33 am
Location: Alberta, Canada

Re: Larger Diatoms Just For Fun

#23 Post by McConkey » Tue Jul 12, 2016 11:10 pm

Kurt, I use Photoshop CS5 but what I did can be done with any version of photoshop.


Reduce noise:
Strength - 9
Preserve detail - 60
reduce colour noise - 90
sharpen detail - 25
Sharpen - 30 x 30

Selective colour:
Blacks - Cyan +7
Yellows - Yellow -100
Cyan - Cyan -100

Shadows/Highlights:
Shadows - 0
Highlights - 5

40% Burn Tool on Diatom

Hue/Saturation:
Hue - (-11)
Saturation -43


- To turn the background to a neutral gray i created a duplicate layer (named gray layer) and with the selective colour adjustment tool, under black...turned down the black until i had a tone i liked.
- I then applied a 15pixel Gaussian Blur to the "Gray" layer and applied a black layer mask (revealing the original image below)
- Using the white paint brush at 90% opacity i "painted" in the gray background on the black layer mask.
- When i got closer to the diatom edge i reduced the opacity to 40%...and then when i got even closer reduced it to 10%

Again, this was just a fun little play around with an already superb image that didn't need "improving" or "altering" in any way, shape, or form. Just a demonstration of post processing.
Karl
AO21 with Canon M3

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#24 Post by KurtM » Wed Jul 13, 2016 12:24 am

Opinions no doubt vary, but here's mine (since it's my image): Post processing is part and parcel of digital imaging, and is not "cheating" in any way, shape, or form. I'm speaking of cropping and adjusting levels and such, not cutting and pasting to make "photo-chopped" images. While I very much appreciate the kind intent when you say my image needed no "improving" or "altering", I gotta say yes, actually it does, and so does any image as far as I'm concerned. When you have a whole world of adjustments to make, there's simply no reason to accept an image straight out of the camera as the best you can possibly offer.

I'm not sure I've taken a picture yet that couldn't be improved by tweaking saturation or contrast, or maybe sharpening, or whatever helps it. And it isn't just a modern thing, top photogs used all sorts of "darkroom magic" to put a polish on their images back in film days.

Karl, I love what you did there; the image with the light background makes the diatom look like it's chrome plated, it really knocks me out, very cool! 8-)

If anyone else cares to take a shot at it, the offer's still open.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Larger Diatoms Just For Fun

#25 Post by 75RR » Thu Jul 14, 2016 12:21 am

Karl, I love what you did there; the image with the light background makes the diatom look like it's chrome plated, it really knocks me out, very cool!
Agree, very nice variation. Nicely sharpened as well.

Image
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
KurtM
Posts: 1753
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Larger Diatoms Just For Fun

#26 Post by KurtM » Thu Jul 14, 2016 1:55 am

Thanks for the reply, 75 - not only was I hoping for more post processing input, but the sharpening demonstration helps bring out some detail that got me all hung up on this particular diatom lately. Some weeks (months?) back I started asking what makes Pinnularia and Navicula so different that they're a whole different genus, especially when some species can look so very similar to those on the opposing team? Well, the answer is (in part) in the stria. Seems Naviculae exhibit areolae in the stria, while Pinularia stria are chambered (thanks exmarine for the clue!). Okay that's a good indicator, just look for the longtitudal lines created by the presence of chambers. But what in the world is going on with chambered stria?!

Seems like every week I'm obsessed with a different diatom, but that's what makes this so much FUN!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

Post Reply