Arcella mitrata

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cuxlander
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Arcella mitrata

#1 Post by cuxlander » Fri Oct 21, 2016 5:02 pm

Hello,
I would like to show you this testate amoeba Arcella mitrata, one of the most beautiful arcellae:

Image

looking from bottom into the mouth with its crenulated rim.

(Müller Biolab-T, Camera Tucsen IS300, Software ISCapture)

Greetings,
Hans
Last edited by cuxlander on Sat Oct 22, 2016 10:44 am, edited 1 time in total.
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vasselle
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Re: Arcella mitrata

#2 Post by vasselle » Fri Oct 21, 2016 5:52 pm

Bonjour
Superbe image
Cordialement seb
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Radazz
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Re: Arcella mitrata

#3 Post by Radazz » Fri Oct 21, 2016 10:40 pm

Wow!
Arnold, Missouri
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JimT
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Re: Arcella mitrata

#4 Post by JimT » Fri Oct 21, 2016 11:11 pm

Wow!
That sums it up for me. Great image.
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Manfred
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Re: Arcella mitrata

#5 Post by Manfred » Sat Oct 22, 2016 5:33 pm

Great image, what magnification, which stacking software to you use?

Regards
Manfred
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75RR
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Re: Arcella mitrata

#6 Post by 75RR » Sat Oct 22, 2016 5:49 pm

Very nice 3D effect! Nice view of the crenulated opening.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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billben74
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Re: Arcella mitrata

#7 Post by billben74 » Sat Oct 22, 2016 9:59 pm

Really fantastic image.
Any preparation details? We love details ;)
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cuxlander
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Re: Arcella mitrata

#8 Post by cuxlander » Mon Oct 24, 2016 1:06 pm

Hello,

I am glad you like the photo. And yes I owe you a few details.
billben: No preparation, the specimen came straight from the pond. I was lucky to get a young one (the older the darker and opaquer) with a tidy test. And I was again lucky to be able to turn it into this position.
I stacked about a dozen frames with Picolay.
Only very little editing, slightly more contrast and sharpening, and I removed the background.
Size of the test about 125 µm.

Here is one focal plane of the same individuum:

Image

The sarcode does not fill the test, but is attached to it by so called epipodia. See the rim of the mouth turned outwards within the convex bottom.

Greetings,
Hans
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75RR
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Re: Arcella mitrata

#9 Post by 75RR » Mon Oct 24, 2016 1:30 pm

And I was again lucky to be able to turn it into this position
How did you turn it and what objective did you use?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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cuxlander
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Re: Arcella mitrata

#10 Post by cuxlander » Mon Oct 24, 2016 1:57 pm

Hello 75RR,
Objective was 40x Achromat (Müller)
I like to use cavity slides, they provide a small aquarium for live organisms, fragile objects do not crush too easily in there, and turning your objects is a bit easier.
Big disadvantage for the photographer: organismes have more space to move, and the extra water layer reduces quality :
Turning is 2% skill and 98% luck. I tip on the cover glass with a needle, caaaarefully push it sideways.
Cheers,
Hans
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75RR
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Re: Arcella mitrata

#11 Post by 75RR » Mon Oct 24, 2016 2:31 pm

Turning is 2% skill and 98% luck
:)
Müller Biolab-T
New microscope?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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cuxlander
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Re: Arcella mitrata

#12 Post by cuxlander » Mon Oct 24, 2016 4:46 pm

No, my old one. I received the Zeiss Junior in September, before that I used the Müller and the Tucsen Camera for several years.
Hans
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gekko
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Re: Arcella mitrata

#13 Post by gekko » Tue Oct 25, 2016 8:59 pm

Lovely image that shows the 3D shape very well.
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