Drosophila melanogaster,fruit fly.

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Seb28
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Drosophila melanogaster,fruit fly.

#1 Post by Seb28 » Sat Dec 10, 2016 6:30 pm

Drosophila melanogaster cleared in KOH,whole mount in glicerine.
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Manfred
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Re: Drosophila melanogaster,fruit fly.

#2 Post by Manfred » Sat Dec 10, 2016 6:47 pm

Great, outstanding images, would be very interesting to know the technic you`re using

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gekko
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Re: Drosophila melanogaster,fruit fly.

#3 Post by gekko » Sat Dec 10, 2016 7:17 pm

Wow! Exceptional work!

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hkv
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Re: Drosophila melanogaster,fruit fly.

#4 Post by hkv » Sat Dec 10, 2016 7:35 pm

Outstanding! Fluorescence?
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Re: Drosophila melanogaster,fruit fly.

#5 Post by zzffnn » Sat Dec 10, 2016 8:23 pm

Wow, great work!

How did you mount such a thick insect, in glycerine, without crashing it, and prevent any subject movement after mounting? Sorry for the newbie question.

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billben74
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Re: Drosophila melanogaster,fruit fly.

#6 Post by billben74 » Sat Dec 10, 2016 10:42 pm

I'll just repeat the others.
Fine work, both in terms of photography and as, zzffnn mentioned, in terms of mounting.

I have tried some insect/arthropod mounting and find it hit and miss.

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75RR
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Re: Drosophila melanogaster,fruit fly.

#7 Post by 75RR » Sat Dec 10, 2016 11:04 pm

Excellent images. Glad you did not just go with the easyzoom - much prefer the others.
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Re: Drosophila melanogaster,fruit fly.

#8 Post by einman » Sat Dec 10, 2016 11:11 pm

Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.

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Re: Drosophila melanogaster,fruit fly.

#9 Post by Seb28 » Sun Dec 11, 2016 3:11 pm

Thank you all for your kind comments.

I begin by placing the specimen in KOH/NaOH solution, heat up to ∼90–100 ◦C,5-10min, preferably in a water bath,then washing in pure water.
The most difficult procedure which is a placement and positioning the insect on the slide.
In the center of a microscope slide I put a drop of pure glycerine,transfer the insect by DIY spatula (bend syringe needle) in to glicerine drop.
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To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick.
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After properly positioning I put very gently the cover slip and sealed with nail varnish
.
einman wrote:Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.
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Re: Drosophila melanogaster,fruit fly.

#10 Post by Seb28 » Sun Dec 11, 2016 3:17 pm

hkv wrote:Outstanding! Fluorescence?
This is not fluorescence,I invert bright field image in Photoshop,and play with the colors.
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75RR
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Re: Drosophila melanogaster,fruit fly.

#11 Post by 75RR » Mon Dec 12, 2016 9:19 pm

To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick.
Pretty neat tools!
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Re: Drosophila melanogaster,fruit fly.

#12 Post by JimT » Mon Dec 12, 2016 10:14 pm

Seb 28, these are all great images and your techniques are very good.

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Re: Drosophila melanogaster,fruit fly.

#13 Post by Rodney » Tue Dec 13, 2016 2:52 am

No doubt those images places you into the world of a fruit fly. The time it takes to do all of this is worth the journey for most of us to view.
Rodney

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Re: Drosophila melanogaster,fruit fly.

#14 Post by billbillt » Tue Dec 13, 2016 6:04 pm

These are exquisite... Very sharp and vibrant colors and clarity...

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Re: Drosophila melanogaster,fruit fly.

#15 Post by einman » Thu Dec 15, 2016 4:09 am

Yes- Exquisite!

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Re: Drosophila melanogaster,fruit fly.

#16 Post by Seb28 » Sat Dec 17, 2016 11:30 am

Thank you all.
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