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Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 6:30 pm
by Seb28
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 6:47 pm
by Manfred
Great, outstanding images, would be very interesting to know the technic you`re using
Regards
Manfred
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 7:17 pm
by gekko
Wow! Exceptional work!
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 7:35 pm
by hkv
Outstanding! Fluorescence?
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 8:23 pm
by zzffnn
Wow, great work!
How did you mount such a thick insect, in glycerine, without crashing it, and prevent any subject movement after mounting? Sorry for the newbie question.
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 10:42 pm
by billben74
I'll just repeat the others.
Fine work, both in terms of photography and as, zzffnn mentioned, in terms of mounting.
I have tried some insect/arthropod mounting and find it hit and miss.
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 11:04 pm
by 75RR
Excellent images. Glad you did not just go with the easyzoom - much prefer the others.
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 10, 2016 11:11 pm
by einman
Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.
Re: Drosophila melanogaster,fruit fly.
Posted: Sun Dec 11, 2016 3:11 pm
by Seb28
Thank you all for your kind comments.
I begin by placing the specimen in KOH/NaOH solution, heat up to ∼90–100 ◦C,5-10min, preferably in a water bath,then washing in pure water.
The most difficult procedure which is a placement and positioning the insect on the slide.
In the center of a microscope slide I put a drop of pure glycerine,transfer the insect by DIY spatula (bend syringe needle) in to glicerine drop.
- IMG_2871.JPG (59.72 KiB) Viewed 6579 times
To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick.
- IMG_2875.JPG (55.31 KiB) Viewed 6579 times
- IMG_2878.JPG (108.68 KiB) Viewed 6579 times
After properly positioning I put very gently the cover slip and sealed with nail varnish
.
einman wrote:Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.
102 images stack,Zeiss CP-achromat 10x/0.25,ring lamp,Canon 50D
Re: Drosophila melanogaster,fruit fly.
Posted: Sun Dec 11, 2016 3:17 pm
by Seb28
hkv wrote:Outstanding! Fluorescence?
This is not fluorescence,I invert bright field image in Photoshop,and play with the colors.
- Drosophila-melanogaster,2,antenna,s.jpg (168.77 KiB) Viewed 6578 times
- Drosophila-melanogaster,2,antenna,,invert..jpg (223.44 KiB) Viewed 6578 times
Re: Drosophila melanogaster,fruit fly.
Posted: Mon Dec 12, 2016 9:19 pm
by 75RR
To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick.
Pretty neat tools!
Re: Drosophila melanogaster,fruit fly.
Posted: Mon Dec 12, 2016 10:14 pm
by JimT
Seb 28, these are all great images and your techniques are very good.
Re: Drosophila melanogaster,fruit fly.
Posted: Tue Dec 13, 2016 2:52 am
by Rodney
No doubt those images places you into the world of a fruit fly. The time it takes to do all of this is worth the journey for most of us to view.
Rodney
Re: Drosophila melanogaster,fruit fly.
Posted: Tue Dec 13, 2016 6:04 pm
by billbillt
These are exquisite... Very sharp and vibrant colors and clarity...
BillT
Re: Drosophila melanogaster,fruit fly.
Posted: Thu Dec 15, 2016 4:09 am
by einman
Yes- Exquisite!
Re: Drosophila melanogaster,fruit fly.
Posted: Sat Dec 17, 2016 11:30 am
by Seb28
Thank you all.
Re: Drosophila melanogaster,fruit fly.
Posted: Sun Oct 04, 2020 3:21 am
by Kevin_r
Hi!
Thank you for such an inspiring subject!
I am wondering if you could help us (the newbies) in step-by-step preparation.
I find it hard to keep the sample in place. Or sometimes, the coverslips just crush the fly.