A couple pictures of a Closterium

[Pat Thielen]

Here are a couple photos of a Closterium; one is taken using oblique phase lighting and the other is dark field. I'm still working on the oblique lighting technique, but I've liked what I've seen so far. I did try taking a stacked image of this guy; I took ten photos total but I didn't like how it came out once I had processed it (it just didn't look real and the edges of the Closterium didn't come out right). As always, let me know what you think of the images -- Any advice for improvement is always greatly appreciated.

[JimT]

I like them both. Could you see the crystal movement in the vacuole?

[zzffnn]

Very nice, Pat!
Have you tried oblique alone. Or using 100x or 40x phase ring with 20x objective, for circular oblique light?

[Pat Thielen]

I like them both. Could you see the crystal movement in the vacuole?

I certainly did! Also, this guy did a slow ninety-degree turn as I watched. It was really quite interesting.

[Pat Thielen]

Very nice, Pat!
Have you tried oblique alone. Or using 100x or 40x phase ring with 20x objective, for circular oblique light?

I haven't but it does sound like an interesting idea. I'm not sure if I can do oblique alone with my microscope; I'll have to do a bit of research first. Thanks for the suggestions!

[mrsonchus]

Interesting and very nice, clear images Pat - nicely done!
John B.

[JimT]

Pat, check out this site from the home page re oblique filters.

http://www.microbehunter.com/oblique-illumination/

Easy to make your own.

Lots of good articles and tutorials available thanks to Oliver.

JimT

[gekko]

Very nice photos.

[Pat Thielen]

Very nice photos.

Thanks!

[Pat Thielen]

Pat, check out this site from the home page re oblique filters.

http://www.microbehunter.com/oblique-illumination/

Easy to make your own.

Lots of good articles and tutorials available thanks to Oliver.

JimT

Thanks for the link -- I'll give this a try when I get a chance. There are some really interesting lighting techniques to try out!

[Pat Thielen]

Interesting and very nice, clear images Pat - nicely done!
John B.

Thanks John!