Oniscus

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Seb28
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Oniscus

#1 Post by Seb28 » Wed Jan 28, 2015 12:15 am

All images with 5x objective,fluorescence light (UV),stained with calcofluor white.1-2 single shots,3-4 stack
In the last two pictures I changed the white balance in my camera.
1.
IMG_2462.jpg
IMG_2462.jpg (71.56 KiB) Viewed 3657 times
2.
IMG_2468.jpg
IMG_2468.jpg (75.46 KiB) Viewed 3657 times
3.
t1.jpg
t1.jpg (57.28 KiB) Viewed 3657 times
4.
t2.jpg-x.jpg
t2.jpg-x.jpg (77.83 KiB) Viewed 3657 times
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D

JimT
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Re: Oniscus

#2 Post by JimT » Wed Jan 28, 2015 12:27 am

That last one is going to give me nightmares! Great images.

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Crater Eddie
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Re: Oniscus

#3 Post by Crater Eddie » Wed Jan 28, 2015 12:59 am

Reminds me a little bit of Pilot on Farscape.
Good pics.
CE
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gekko
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Re: Oniscus

#4 Post by gekko » Thu Jan 29, 2015 1:13 am

Very beautiful images! How do you apply the fluorescent stain and for how long?

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vasselle
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Re: Oniscus

#5 Post by vasselle » Thu Jan 29, 2015 9:22 am

Bonjour
Superbes photos.
J'adore les deux dernières elles sont magnifiques.
Merci pour le partage.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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Seb28
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Re: Oniscus

#6 Post by Seb28 » Thu Jan 29, 2015 12:27 pm

Thank you.
gekko wrote:How do you apply the fluorescent stain and for how long?
When staining small anthropds (ex.copoped) I put them in to staining solution for a few hours or overnight.
With much larger anthropods ,you need to put them in KOH solution first (some times boiling few minutes) and rinse,then put to the staining solution overnight and rinse again.I just starting adventure with fluorescent staining, it is a good fun.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D

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gekko
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Re: Oniscus

#7 Post by gekko » Sun Feb 01, 2015 2:05 pm

Seb28 wrote:Thank you.
gekko wrote:How do you apply the fluorescent stain and for how long?
When staining small anthropds (ex.copoped) I put them in to staining solution for a few hours or overnight.
With much larger anthropods ,you need to put them in KOH solution first (some times boiling few minutes) and rinse,then put to the staining solution overnight and rinse again.I just starting adventure with fluorescent staining, it is a good fun.
Thank you for the details.

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hkv
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Re: Oniscus

#8 Post by hkv » Sun Feb 01, 2015 4:59 pm

Where can one buy Calcofluor? I need some. Great result!
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