Copepod (Stack and stitch) in fluorescence

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hkv
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Copepod (Stack and stitch) in fluorescence

#1 Post by hkv » Fri May 05, 2017 12:11 pm

Enough with the algae for a while so here comes a marine copepod shot with an UplanSAPO 10X objective. Stacked in two piles and then stitched in photoshop. Shot using fluorescence illumination with a mercury light source at a wavelength around 400-440 nm. I previously posted images of this copepod using the 4X objective, but here the resolution is much better. The inside of the copepod is red due to the algae he has been eating.

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rnabholz
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Re: Copepod (Stack and stitch) in fluorescence

#2 Post by rnabholz » Fri May 05, 2017 12:28 pm

Oh my goodness, that is a fascinating and beautiful image!

Don't know much about flourescence, and don't see it deployed often, but this is really wonderful.

Congratulations

Rod

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Re: Copepod (Stack and stitch) in fluorescence

#3 Post by kit1980 » Fri May 05, 2017 4:08 pm

Beautiful!
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Re: Copepod (Stack and stitch) in fluorescence

#4 Post by billbillt » Fri May 05, 2017 5:22 pm

ASTONISHING!... What a wonderful image!.. perfectly clear and sharp!...

BillT

charlie g
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Re: Copepod (Stack and stitch) in fluorescence

#5 Post by charlie g » Fri May 05, 2017 6:59 pm

Gorgeous image, hkv! About what region of this crusteacean did you make the stich of the two stacks?

As your resolution is great with 10X apo..could one make a stich of say four stacks for use of a 40X apo? Or does each stich subtely (?sp?) distort the reality of the body plan? I ask as kindly in this forum, a querry for advice I made posting segments of a 2-3 mm long ciliate (Spirostomum sp.), well I was given immediate and specific stich software suggestions.

I,m curious if stich image processing can be faithful to body plans..or is there always a tiny region that is altered or omitted in the end result. This image of yours would go well on a wall in the study, thanks again, hkv! charlie guevara
Last edited by charlie g on Sat May 06, 2017 12:29 am, edited 1 time in total.

JimT
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Re: Copepod (Stack and stitch) in fluorescence

#6 Post by JimT » Fri May 05, 2017 10:34 pm

Oh my goodness, that is a fascinating and beautiful image!
Rod said it all. Beautiful!

Rodney
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Re: Copepod (Stack and stitch) in fluorescence

#7 Post by Rodney » Sat May 06, 2017 1:45 am

Dang, that looks good. If you find any microscopic water mites around I would like to take a look with that procedure.
Rodney

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Re: Copepod (Stack and stitch) in fluorescence

#8 Post by zzffnn » Sat May 06, 2017 2:31 am

Very impressive!
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hkv
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Re: Copepod (Stack and stitch) in fluorescence

#9 Post by hkv » Sat May 06, 2017 11:41 am

Thanks all for you comments!
charlie g wrote:Gorgeous image, hkv! About what region of this crusteacean did you make the stich of the two stacks?

As your resolution is great with 10X apo..could one make a stich of say four stacks for use of a 40X apo? Or does each stich subtely (?sp?) distort the reality of the body plan? I ask as kindly in this forum, a querry for advice I made posting segments of a 2-3 mm long ciliate (Spirostomum sp.), well I was given immediate and specific stich software suggestions.

I,m curious if stich image processing can be faithful to body plans..or is there always a tiny region that is altered or omitted in the end result. This image of yours would go well on a wall in the study, thanks again, hkv! charlie guevara
The stitch is close to the left arm that sticks out. Just below is stitch 1 and the upper part in stitch 2.

10X is just about as high I can get on this subject due to its thickness. The cover glass has small distances to avoid crushing the copepod so the water film is rather deep. The 20X and the 40X does not have enough working distance to allow it to focus before it hits the cover glass. Perhaps with the 20X I could get the top of the animal in focus, but not reach through the whole depth.
Flickr: https://www.flickr.com/photos/micromundus
Youtube: https://www.youtube.com/channel/UC0UdYN ... YH_litDZjA
Instagram: https://www.instagram.com/micromundusphotography
Olympus BX51 | Olympus SZ4045 | Carl Zeiss Jena Sedival | Swift FM-31

Manfred
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Re: Copepod (Stack and stitch) in fluorescence

#10 Post by Manfred » Sat May 06, 2017 5:42 pm

Outstanding image, wonderful, thanks for sharing

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