MicrobeHunter.com Microscopy Forum

Enough with the algae for a while so here comes a marine copepod shot with an UplanSAPO 10X objective. Stacked in two piles and then stitched in photoshop. Shot using fluorescence illumination with a mercury light source at a wavelength around 400-440 nm. I previously posted images of this copepod using the 4X objective, but here the resolution is much better. The inside of the copepod is red due to the algae he has been eating.

Oh my goodness, that is a fascinating and beautiful image!

Don't know much about flourescence, and don't see it deployed often, but this is really wonderful.

Congratulations

Rod

Beautiful!

ASTONISHING!... What a wonderful image!.. perfectly clear and sharp!...

BillT

Gorgeous image, hkv! About what region of this crusteacean did you make the stich of the two stacks?

As your resolution is great with 10X apo..could one make a stich of say four stacks for use of a 40X apo? Or does each stich subtely (?sp?) distort the reality of the body plan? I ask as kindly in this forum, a querry for advice I made posting segments of a 2-3 mm long ciliate (Spirostomum sp.), well I was given immediate and specific stich software suggestions.

I,m curious if stich image processing can be faithful to body plans..or is there always a tiny region that is altered or omitted in the end result. This image of yours would go well on a wall in the study, thanks again, hkv! charlie guevara

Oh my goodness, that is a fascinating and beautiful image!

Rod said it all. Beautiful!

Dang, that looks good. If you find any microscopic water mites around I would like to take a look with that procedure.
Rodney

Very impressive!

Thanks all for you comments!

charlie g wrote:Gorgeous image, hkv! About what region of this crusteacean did you make the stich of the two stacks?

As your resolution is great with 10X apo..could one make a stich of say four stacks for use of a 40X apo? Or does each stich subtely (?sp?) distort the reality of the body plan? I ask as kindly in this forum, a querry for advice I made posting segments of a 2-3 mm long ciliate (Spirostomum sp.), well I was given immediate and specific stich software suggestions.

I,m curious if stich image processing can be faithful to body plans..or is there always a tiny region that is altered or omitted in the end result. This image of yours would go well on a wall in the study, thanks again, hkv! charlie guevara

The stitch is close to the left arm that sticks out. Just below is stitch 1 and the upper part in stitch 2.

10X is just about as high I can get on this subject due to its thickness. The cover glass has small distances to avoid crushing the copepod so the water film is rather deep. The 20X and the 40X does not have enough working distance to allow it to focus before it hits the cover glass. Perhaps with the 20X I could get the top of the animal in focus, but not reach through the whole depth.

Outstanding image, wonderful, thanks for sharing