Prepared slides - Pine studies

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JuiceKing
Posts: 10
Joined: Sat May 27, 2017 2:37 am

Prepared slides - Pine studies

#1 Post by JuiceKing » Mon May 29, 2017 3:25 pm

Is there a more photogenic plant? Who knew?

Images link to larger/full-size images.

Image
Pine root c.s. (Amscope PS100A - #66 prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um

Image
Pine Stem c.s. (Amscope PS100A #67 prepared slide)
Stacked 10 images Toupview EDF
Nikon Plan Apo 4/0.20
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um

Image
Pine Stem c.s. (Amscope PS100A #67 prepared slide)
Nikon Plan Apo 10/0.45
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um

Image
Pine Leaf c.s. (Amscope PS100A #65 prepared slide)
Stitched 3 images, Toupview EDF
Nikon Plan Apo 10/0.45
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um

Image
Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
2.5x projection eyepiece into Canon 5DMk2

Image
Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 10/0.45, Achr @0.35
2.5x projection eyepiece into Canon 5DMk2

Image
Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 40/0.95, Achr @0.70
2.5x projection eyepiece into Canon 5DMk2

Image
Pine root c.s. (Carton prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
2.5x projection eyepiece into Canon 5DMk2
Damage to cover slip causes fuzziness and shading on 4PM central area

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vasselle
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Location: France

Re: Prepared slides - Pine studies

#2 Post by vasselle » Tue May 30, 2017 5:32 pm

Bonjour
Superbes photos et jolie travail
Bravo.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Prepared slides - Pine studies

#3 Post by 75RR » Tue May 30, 2017 6:00 pm

Plant sections can be quite beautiful.
Be aware that more than one forum member has succumbed to microtomeitis. Full recoveries are rare.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

JuiceKing
Posts: 10
Joined: Sat May 27, 2017 2:37 am

Re: Prepared slides - Pine studies

#4 Post by JuiceKing » Wed May 31, 2017 12:24 am

Thank you. One after the next, these botanical sections are stunning.

I am intrigued but a little intimidated by the microtome step. There are too many enthusiastic posts about huge machines that will certainly push my poor, patient better half past her limit. Are there hand-units worth a serious try?

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75RR
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Location: Estepona, Spain

Re: Prepared slides - Pine studies

#5 Post by 75RR » Wed May 31, 2017 12:48 am

Are there hand-units worth a serious try?
They tend to be a bit overpriced and not that effective.
You could try two old style razor-blades sandwiching a third (broken in half) to start off.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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mrsonchus
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Location: Cumbria, UK

Re: Prepared slides - Pine studies

#6 Post by mrsonchus » Wed May 31, 2017 12:18 pm

Hi, I would definitely agree with 75' here. The hand-microtomes (AKA 'bench' microtomes) are really little better if at all than the razor-blade-in-hand method in my experience.

The "huge machines" require wax-embedded and fully processed tissue to section in the realm of anything really, from about 20µ right down to a terrifyingly-thin 1µ. These really do require a serious amount of time, effort and indeed investment, as with the purchase of such a machine as a Shandon rotary the entire histological microtechnique must be mastered.

Jim produces the nicest hand-section I've seen, and he I believe uses a simple technique with the application of a large amount of skill, selective-judgement and care.

Good luck, you've a lot of enjoyment waiting for you! :)
John B

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