MicrobeHunter.com Microscopy Forum

Is there a more photogenic plant? Who knew?

Images link to larger/full-size images.


Pine root c.s. (Amscope PS100A - #66 prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um


Pine Stem c.s. (Amscope PS100A #67 prepared slide)
Stacked 10 images Toupview EDF
Nikon Plan Apo 4/0.20
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um


Pine Stem c.s. (Amscope PS100A #67 prepared slide)
Nikon Plan Apo 10/0.45
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um


Pine Leaf c.s. (Amscope PS100A #65 prepared slide)
Stitched 3 images, Toupview EDF
Nikon Plan Apo 10/0.45
0.5x reducer lens
Image sensor: Aptina 1/2.3inch color CMOS, 4912x3684 pixels; Pixel size: 1.25 um x 1.25 um


Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
2.5x projection eyepiece into Canon 5DMk2


Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 10/0.45, Achr @0.35
2.5x projection eyepiece into Canon 5DMk2


Pine Cone - Female, l.s. (Amscope PS100B - #66 prepared slide)
Nikon Plan Apo 40/0.95, Achr @0.70
2.5x projection eyepiece into Canon 5DMk2


Pine root c.s. (Carton prepared slide)
Nikon Plan Apo 4/0.20, Achr flip top open
2.5x projection eyepiece into Canon 5DMk2
Damage to cover slip causes fuzziness and shading on 4PM central area

Bonjour
Superbes photos et jolie travail
Bravo.
Cordialement seb

Plant sections can be quite beautiful.
Be aware that more than one forum member has succumbed to microtomeitis. Full recoveries are rare.

Thank you. One after the next, these botanical sections are stunning.

I am intrigued but a little intimidated by the microtome step. There are too many enthusiastic posts about huge machines that will certainly push my poor, patient better half past her limit. Are there hand-units worth a serious try?

Are there hand-units worth a serious try?

They tend to be a bit overpriced and not that effective.
You could try two old style razor-blades sandwiching a third (broken in half) to start off.

Hi, I would definitely agree with 75' here. The hand-microtomes (AKA 'bench' microtomes) are really little better if at all than the razor-blade-in-hand method in my experience.

The "huge machines" require wax-embedded and fully processed tissue to section in the realm of anything really, from about 20µ right down to a terrifyingly-thin 1µ. These really do require a serious amount of time, effort and indeed investment, as with the purchase of such a machine as a Shandon rotary the entire histological microtechnique must be mastered.

Jim produces the nicest hand-section I've seen, and he I believe uses a simple technique with the application of a large amount of skill, selective-judgement and care.

Good luck, you've a lot of enjoyment waiting for you!