Degassing plant tissues for better processing/mounting the easy way

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mrsonchus
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Degassing plant tissues for better processing/mounting the easy way

#1 Post by mrsonchus » Fri Jun 09, 2017 11:33 pm

Hi all, some of you may have seen my newly posted thread starting an adventure to ultimately produce some root-tip mitosis and anatomy slides. This thread is just a little video to show how to remove the air from within tissues that 'need a little help' to fully infiltrate with liquid, be it an alcohol dehydrant, water-mount or even permanent mountant, to ensure no post-mount air bubble will appear.

In this example one of the sprouted seed types of 'the other thread' needed the application the release of a vacuum to aid air removal as the tissues were being placed into fixative at the very start of the process....



If you need any details, just let me know - this technique is great for tissue that is only to be water-mounted temporarily too!

The cheap car brake bleeding pump seen in the video was suggested by another forum member - up until then I had been using a big syringe and fish-tank airline valves - which worked fine but not as efficiently as this method!

John B. :D :D :)
John B

steviem
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Joined: Tue Jul 25, 2017 10:44 am

Re: Degassing plant tissues for better processing/mounting the easy way

#2 Post by steviem » Fri Jul 28, 2017 4:04 pm

Hi, another possible solution for you is to use a fishermans "pellet pump" readily available on ebay-about £15

billbillt
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Joined: Mon Nov 10, 2014 10:01 pm

Re: Degassing plant tissues for better processing/mounting the easy way

#3 Post by billbillt » Fri Jul 28, 2017 4:40 pm

steviem wrote:Hi, another possible solution for you is to use a fishermans "pellet pump" readily available on ebay-about £15
I believe Mr. John B. uses a device such as this:

http://www.ebay.com/itm/Bstean-100ml-La ... 1438.l2649


Regards,
BillT

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mrsonchus
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Re: Degassing plant tissues for better processing/mounting the easy way

#4 Post by mrsonchus » Fri Jul 28, 2017 6:05 pm

Aha! Now, that was the v1; I now use a car brake-bleeding pump which has an open/closed valve and a guage built in - even enables a quantified vacuum to be applied as an aid to consistency.

This is the one I now use - this type of device was recommended to me by someone here in the forum, trouble is I forget who it was.

This is about £12 from e-bay and works perfectly....
vacuum.jpg
vacuum.jpg (14.67 KiB) Viewed 1079 times
John B :)
John B

billbillt
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Re: Degassing plant tissues for better processing/mounting the easy way

#5 Post by billbillt » Fri Jul 28, 2017 9:10 pm

Thanks for the update, my friend!....

BillT

Moose
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Re: Degassing plant tissues for better processing/mounting the easy way

#6 Post by Moose » Sat Jul 29, 2017 2:41 am

I love it. I have worked in the microscopy field for almost 40 years. I have seen attachments, devices, etc cost hundreds or thousands of dollars. It is so nice to see someone come up with simple attachments to the job of expensive equipment.
Moose
Olympus BHS Trinocular with polarizer/analyzer
Canon 5DMark4 camera on trinocular head with photomicro L adapter.

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