Diatom (another)

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Diatom (another)

#1 Post by gekko » Mon Feb 02, 2015 11:37 pm

Another diatom from the muddy water of the pothole in the yard. I intended to take a focus stack, but it was moving all the time so I did the best I could with a single shot.

1. Bright field, 20x objective:
Image

2. DIC, 20x objective:
Image

User avatar
Crater Eddie
Posts: 1829
Joined: Wed Nov 12, 2014 4:39 pm
Location: Illinois USA

Re: Diatom (another)

#2 Post by Crater Eddie » Tue Feb 03, 2015 12:13 am

To me these look very good for one shot each.
CE
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)

User avatar
lorez
Posts: 735
Joined: Wed Dec 17, 2014 1:48 am

Re: Diatom (another)

#3 Post by lorez » Tue Feb 03, 2015 1:41 am

Gekko,

I agree with CE. Very nice. You have mastered the art of the single exposure. I like both photos and especially the DIC, even though it may give some false relief. It makes the lipid globules much more apparent. Three cheers for a job well done.

lorez

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Diatom (another)

#4 Post by gekko » Tue Feb 03, 2015 3:59 am

CE and lorez, thank you very much for your kind (actually too kind) comments. I must admit that I've not had good results with focus stacks even when the diatoms were stationary. True the DIC image does highlight the lipid globules but I think I should have toned down the DIC effect somewhat. Thanks again.

User avatar
vasselle
Posts: 2763
Joined: Sat Oct 25, 2014 5:32 pm
Location: France

Re: Diatom (another)

#5 Post by vasselle » Tue Feb 03, 2015 3:14 pm

Bonjour Gekko
Belles séries images
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Diatom (another)

#6 Post by gekko » Tue Feb 03, 2015 5:56 pm

seb, thank you again for your kind comment.

User avatar
hkv
Posts: 967
Joined: Sun Nov 02, 2014 8:57 pm
Location: Sweden
Contact:

Re: Diatom (another)

#7 Post by hkv » Tue Feb 03, 2015 7:14 pm

I like the last one, which almost look like a school book illustration. Looks like a painting almost. Nice effect. Is that achieved in the post processing or a "DIC effect"? I have not experienced such effect on my DIC setup (yet)

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Diatom (another)

#8 Post by gekko » Tue Feb 03, 2015 8:43 pm

hkv wrote:I like the last one, which almost look like a school book illustration. Looks like a painting almost. Nice effect. Is that achieved in the post processing or a "DIC effect"? I have not experienced such effect on my DIC setup (yet)
Thank you, khv: I very much appreciate any feedback. I think I have overdone the DIC (set the prism for maximum effect) which gives it an exaggerated (fake) 3D effect. But I'm not sure what you are referring to exactly. On my microscope, the DIC prism incorporates a retardation plate, so as I move the slider, the color changes. I usually try to set it for a neutral gray background, but in the image captured by the camera, the background comes out as a deep cyan; I don't know why. In editing, I found that if I use the eyedropper on the background to render it gray, the colors of the diatom (or whatever the object is) also change accordingly (as can be expected). On the other hand, if I desaturate the cyan background color only, then only the background changes to gray, the colors of the object remain unchanged (I guess unless the object contains cyan color), so that is what I did here. After that, I adjusted levels, contrast, resized the image for posting, and sharpened it. But the first step is: in camera raw (I use Adobe Elements) I adjust the exposure slightly if necessary, then reduce the noise, then open the image in the editor where I may reduce noise further if necessary. I sometimes attempt to (partially or completely) clean the background when there is "junk" or out-of-focus blobs, depending on whether I think it is distracting or I leave it in if I think it may give "context" (or if I am too lazy). Sometimes the image sort of looks like a water color painting if I use high ISO and then overdo the noise reduction, but in this case I used a low ISO (200) so I don't think that could explain what you describe. Perhaps I oversharpened it? I don't really know. To me the image looks rather "harsh" which I attribute to the strong DIC setting I used. Does any of this explain what you see? Thanks again.
One last possibility: "empty magnification". If I view the image smaller, it looks better to my eyes. I've reduced the size and posted again below: does that help? Thanks again.

Image

Post Reply