Filamentous algae

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Seb28
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Filamentous algae

#1 Post by Seb28 » Wed Feb 04, 2015 10:17 am

Obj.Nikon 20/0.50

autofluo
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IMG_1228.jpg (89.41 KiB) Viewed 1671 times
fluo UV
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IMG_2835.jpg (162.71 KiB) Viewed 1671 times
fluo B
IMG_2836.jpg
IMG_2836.jpg (137.44 KiB) Viewed 1669 times
fluo UV+B
vbc.jpg
vbc.jpg (133.87 KiB) Viewed 1669 times
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D

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75RR
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Re: Filamentous algae

#2 Post by 75RR » Wed Feb 04, 2015 11:00 am

Love the effects of epifluorescence.
autofluo
fluo UV
fluo B
fluo UV + B

First one is the effect I am more familiar with.
How many flou's are there?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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Seb28
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Re: Filamentous algae

#3 Post by Seb28 » Wed Feb 04, 2015 11:11 am

Thank you 75RR

There are four fluorescent exitations: UV-ultra violet,V-fiolet,B-blue and G-green.You can also marge them together.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D

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gekko
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Re: Filamentous algae

#4 Post by gekko » Wed Feb 04, 2015 11:24 am

Wonderful images! By autofluo I guess you mean autofluorescence (before you add the fluo stain); is that right? What excitation wavelength do you use with autofluo?

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Seb28
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Re: Filamentous algae

#5 Post by Seb28 » Wed Feb 04, 2015 11:52 am

Thank you Gekko.
Yes,you're right.
Autofluorescence is the natural emission of light, you can get the autofluorescence on each set(UV,V,B,G) it all depends on the specimen you are watching,for example chlorophyll emit autoflurescence on B(blue excitation) filter set.
Mostly for autofluorescence i use:
UV:
-excitation filter 365/50nm
-dichroic mirror 395nm
-emission filter 445/50nm
B:
-excitation filter 546/10nm
-dichroic mirror 565nm
-emission filter 605/55
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D

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Crater Eddie
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Re: Filamentous algae

#6 Post by Crater Eddie » Wed Feb 04, 2015 2:11 pm

Shedding a new light on a favorite subject. Very interesting.
CE
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hkv
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Re: Filamentous algae

#7 Post by hkv » Wed Feb 04, 2015 8:24 pm

Great images! I need to start using my fluorescence equipment as well. Never used it yet...

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vasselle
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Re: Filamentous algae

#8 Post by vasselle » Thu Feb 05, 2015 9:31 am

Hello
Superbes séries images.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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