Page 1 of 1
Filamentous algae
Posted: Wed Feb 04, 2015 10:17 am
by Seb28
Obj.Nikon 20/0.50
autofluo
- IMG_1228.jpg (89.41 KiB) Viewed 3767 times
fluo UV
- IMG_2835.jpg (162.71 KiB) Viewed 3767 times
fluo B
- IMG_2836.jpg (137.44 KiB) Viewed 3765 times
fluo UV+B
- vbc.jpg (133.87 KiB) Viewed 3765 times
Re: Filamentous algae
Posted: Wed Feb 04, 2015 11:00 am
by 75RR
Love the effects of epifluorescence.
autofluo
fluo UV
fluo B
fluo UV + B
First one is the effect I am more familiar with.
How many flou's are there?
Re: Filamentous algae
Posted: Wed Feb 04, 2015 11:11 am
by Seb28
Thank you 75RR
There are four fluorescent exitations: UV-ultra violet,V-fiolet,B-blue and G-green.You can also marge them together.
Re: Filamentous algae
Posted: Wed Feb 04, 2015 11:24 am
by gekko
Wonderful images! By autofluo I guess you mean autofluorescence (before you add the fluo stain); is that right? What excitation wavelength do you use with autofluo?
Re: Filamentous algae
Posted: Wed Feb 04, 2015 11:52 am
by Seb28
Thank you Gekko.
Yes,you're right.
Autofluorescence is the natural emission of light, you can get the autofluorescence on each set(UV,V,B,G) it all depends on the specimen you are watching,for example chlorophyll emit autoflurescence on B(blue excitation) filter set.
Mostly for autofluorescence i use:
UV:
-excitation filter 365/50nm
-dichroic mirror 395nm
-emission filter 445/50nm
B:
-excitation filter 546/10nm
-dichroic mirror 565nm
-emission filter 605/55
Re: Filamentous algae
Posted: Wed Feb 04, 2015 2:11 pm
by Crater Eddie
Shedding a new light on a favorite subject. Very interesting.
CE
Re: Filamentous algae
Posted: Wed Feb 04, 2015 8:24 pm
by hkv
Great images! I need to start using my fluorescence equipment as well. Never used it yet...
Re: Filamentous algae
Posted: Thu Feb 05, 2015 9:31 am
by vasselle
Hello
Superbes séries images.
Cordialement seb