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Filamentous algae

Posted: Wed Feb 04, 2015 10:17 am
by Seb28
Obj.Nikon 20/0.50

autofluo
IMG_1228.jpg
IMG_1228.jpg (89.41 KiB) Viewed 1735 times
fluo UV
IMG_2835.jpg
IMG_2835.jpg (162.71 KiB) Viewed 1735 times
fluo B
IMG_2836.jpg
IMG_2836.jpg (137.44 KiB) Viewed 1733 times
fluo UV+B
vbc.jpg
vbc.jpg (133.87 KiB) Viewed 1733 times

Re: Filamentous algae

Posted: Wed Feb 04, 2015 11:00 am
by 75RR
Love the effects of epifluorescence.
autofluo
fluo UV
fluo B
fluo UV + B

First one is the effect I am more familiar with.
How many flou's are there?

Re: Filamentous algae

Posted: Wed Feb 04, 2015 11:11 am
by Seb28
Thank you 75RR

There are four fluorescent exitations: UV-ultra violet,V-fiolet,B-blue and G-green.You can also marge them together.

Re: Filamentous algae

Posted: Wed Feb 04, 2015 11:24 am
by gekko
Wonderful images! By autofluo I guess you mean autofluorescence (before you add the fluo stain); is that right? What excitation wavelength do you use with autofluo?

Re: Filamentous algae

Posted: Wed Feb 04, 2015 11:52 am
by Seb28
Thank you Gekko.
Yes,you're right.
Autofluorescence is the natural emission of light, you can get the autofluorescence on each set(UV,V,B,G) it all depends on the specimen you are watching,for example chlorophyll emit autoflurescence on B(blue excitation) filter set.
Mostly for autofluorescence i use:
UV:
-excitation filter 365/50nm
-dichroic mirror 395nm
-emission filter 445/50nm
B:
-excitation filter 546/10nm
-dichroic mirror 565nm
-emission filter 605/55

Re: Filamentous algae

Posted: Wed Feb 04, 2015 2:11 pm
by Crater Eddie
Shedding a new light on a favorite subject. Very interesting.
CE

Re: Filamentous algae

Posted: Wed Feb 04, 2015 8:24 pm
by hkv
Great images! I need to start using my fluorescence equipment as well. Never used it yet...

Re: Filamentous algae

Posted: Thu Feb 05, 2015 9:31 am
by vasselle
Hello
Superbes séries images.
Cordialement seb