Rotaria, amoeba, diatom

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c-krebs
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Rotaria, amoeba, diatom

#1 Post by c-krebs » Tue Sep 05, 2017 10:11 pm

Hello again all. Was reading through the various "boards", learned a few new things and thought I would contribute a few new images.
I haven't had much scope time this summer but I kept an outdoor "tank" which proved to be decidedly non-productive. But I did find a few nice subjects

Here is a Rotaria (Bdelloid rotifer) actively "feeding", a testate amoeba, and a cruising diatom (liked the chloroplasts appearance).



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KurtM
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Re: Rotaria, amoeba, diatom

#2 Post by KurtM » Wed Sep 06, 2017 12:25 am

Absolutely breathtaking, as always. :shock:
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Rodney
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Re: Rotaria, amoeba, diatom

#3 Post by Rodney » Wed Sep 06, 2017 1:06 am

Those are really nice, I like #1 and 3 the best; are those red spots in the first one some type of eye spots?
Rodney

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IanW
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Re: Rotaria, amoeba, diatom

#4 Post by IanW » Wed Sep 06, 2017 5:41 am

No 4 for simplicity and balance.
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75RR
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Re: Rotaria, amoeba, diatom

#5 Post by 75RR » Wed Sep 06, 2017 6:14 am

Lovely photographs. The testate amoeba image shows just why they are so fascinating.
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Francisco
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Re: Rotaria, ameba, diatomea

#6 Post by Francisco » Wed Sep 06, 2017 3:30 pm

Very very nice

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billben74
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Re: Rotaria, amoeba, diatom

#7 Post by billben74 » Thu Sep 07, 2017 8:15 pm

All fantastic. Such wonderful detail on the bdelloid. Always a real treat to see your posts.

JimT
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Re: Rotaria, amoeba, diatom

#8 Post by JimT » Thu Sep 07, 2017 9:05 pm

C-krebs, we want more scope time. One and three are truly fantastic (two and four aren't bad either).

What technique for the rotifer?

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cuxlander
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Re: Rotaria, amoeba, diatom

#9 Post by cuxlander » Sun Sep 10, 2017 9:57 am

Hello Charles,
the rotifer and the diatom are splendid, I assume DIC?
Have you been able to identify the amoeba? The position makes identification difficult, could be a Lesquereusia??
Cheers,
Hans

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c-krebs
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Re: Rotaria, amoeba, diatom

#10 Post by c-krebs » Sun Sep 10, 2017 9:52 pm

Thanks for the kind comments.
JimT wrote:What technique for the rotifer?
All these are DIC, with a "basic" wet mount. One of the more tricky aspects with the rotifers is that often they "sway" back and forth and up and down while "feeding". Since the NA of the objectives used is pretty high (0.70 for the 20X and 40/0.95) you need to find a specimen that is pretty close to the cover slip and time your shot so it has moved as close to the cover as possible. Otherwise you take too large a quality hit due to spherical aberration. The adjustment collar of the 40X would be helpful if the subject were still in the water but often it is constantly moving up and down.
Rodney wrote:are those red spots in the first one some type of eye spots?
Rodney
Yes those are two prominent eye-spots. In Rotaria they located on the "rostrum" as seen here. With a somewhat similar Bdelloid rotifer, Philodina, they are usually seen lower in the body, just above the mastax.
cuxlander wrote:I assume DIC?
Have you been able to identify the amoeba? The position makes identification difficult, could be a Lesquereusia??
Cheers,
Hans
My impression was that it looked a great deal like a Lesquereusia (Lesquereusia modesta?). But I don't know. I probably should have followed it around to get some different views.

charlie g
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Re: Rotaria, amoeba, diatom

#11 Post by charlie g » Mon Sep 11, 2017 4:42 pm

Thanks, Charles, for sharing your beautiful and exacting image captures. I especially like your rotifers crisp depiction of it's flame cells! Charlie guevara

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Re: Rotaria, amoeba, diatom

#12 Post by billbillt » Mon Sep 11, 2017 7:41 pm

I don't think it would be possible to improve on any photo you post.. Incredible!...

BillT

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Johann
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Re: Rotaria, amoeba, diatom

#13 Post by Johann » Thu Sep 14, 2017 5:21 pm

Absolutely amazing - always a pleasure looking at your work!
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vasselle
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Re: Rotaria, amoeba, diatom

#14 Post by vasselle » Tue Sep 19, 2017 12:10 am

Bonjour
Superbes images du grand art
Cordialement seb
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