Fluorescent microscopy

[MicroMacro]

Anyone do any live cell fluorescent microscopy?

It would be awesome to see cell mitosis under our fluoro scopes, this is so cool!!!
1) What fluorophore do you think he used to stain the DNA orange here?
https://www.youtube.com/watch?v=P7m3WfzgZdI

This one is cool too, you can see your white blood cells, the nucleus and DNA packets in there so nicely.
2) What fluorophore do you think he used to stain the blood here?
https://www.youtube.com/watch?v=jnnl9f9b1Vg


This one is way cool, but it looks like it is beyond my fluoro scope reach, as it requires software.
https://www.youtube.com/watch?v=5_bCuOr0Nuk

[MicroMacro]

Also, is there a thread or a website with a good fluorescent microscopy forum?

[billbillt]

Hello,

At one time I had a great interest in fluorescent microscopy, but the urge has waned.. I was looking into producing some kind of an LED system where the light frequency could be changed to match what is produced with the expensive filter cubes that seem to be required for this..

BillT

[zzffnn]

1) that was done by professional genetic engineering (producing an artificial fluorescence fusion protein). Not possible for hobbyists.

2) possible for hobbyists, if you know exactly what to buy and where to buy them. But expect to pay at least $500 USD for a working rig, if you can get that lucky. If you don't already know where and what to buy, or are not as lucky, you may have to spend at least $1500.

Many fluorescence dyes are expensive and toxic too. I suggest doing auto fluorescence, under UV or royal blue light.

[billbillt]

Here is a link to a DIY setup that was done somewhat on the cheap.. Take note of the photo that shows the wavelength of the LED's in this setup.. Of course laboratory results would not be possible at home, but I would expect to get similar results as this...

http://www.microscopy-uk.org.uk/mag/ind ... luoro.html

BillT

[billbillt]

LED fluorescence microscopy in theory and practice

More DIY info for fluorescence microscopy ..

https://web.archive.org/web/20071012205 ... tures.html

BillT