Cross section of aneedle of Picea spec.

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Manfred
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Cross section of aneedle of Picea spec.

#1 Post by Manfred » Tue Feb 10, 2015 7:00 pm

Dear all,
motivated by the section of a pine needle shown by Rod (rnabholz) I tried today the same.
Believe me - its a real challenge.
The section was stained with Wacker FCA.
In picture 2 I try to explain the details, unfortunately only in German (my English is too poor :oops: )
Any comments are welcome, thank you for your kind attention.
Kiefer.jpg
Kiefer.jpg (286.42 KiB) Viewed 4983 times
Kiefer - mit Beschriftung.jpg
Kiefer - mit Beschriftung.jpg (278.63 KiB) Viewed 4983 times
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Kiefer - mit Beschriftung.jpg
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rnabholz
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Re: Cross section of aneedle of Picea spec.

#2 Post by rnabholz » Tue Feb 10, 2015 8:09 pm

Perhaps it is my fresh experience, and less than optimal results speaking, but this is ABSOLUTELY FANTASTIC!!

I had planned to take another run at this subject tonight, but your shot may have defined the genre for all time.

Anything I might post will look like a mud pie now! ;^)

Would love to hear more about the technical details.

Rod

Manfred
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Re: Cross section of aneedle of Picea spec.

#3 Post by Manfred » Tue Feb 10, 2015 8:21 pm

Hello Rod,
thank you for your nice comment.
Most important is to try again and again, also my first attempts were really disatisfying.
Most of my equipment is homemade (microtome, razorblade holder).
To achieve this image I did several dozens of sections, only a few of them were thin enough (ca. 50microns) to allow staining.
Go ahead this way, you will see, your sections will improve day by day.
Warmest regards
Manfred

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mrsonchus
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Re: Cross section of aneedle of Picea spec.

#4 Post by mrsonchus » Tue Feb 10, 2015 9:22 pm

rnabholz wrote:Perhaps it is my fresh experience, and less than optimal results speaking, but this is ABSOLUTELY FANTASTIC!!

I had planned to take another run at this subject tonight, but your shot may have defined the genre for all time.

Anything I might post will look like a mud pie now! ;^)

Would love to hear more about the technical details.

Rod
Hey you've made a great start yourself - I've not even produced anything that's not too bad to post and I've been
trying for a couple of months now. Your technique will definitely get better the more you practice.
I use images from others as my targets to achieve and am slowly making progress too.

Have another go, and then another - it's a great feeling when you see your own progress, especially when you
move into making permanently mounted slides for your own collection.

John
John B

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mrsonchus
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Re: Cross section of aneedle of Picea spec.

#5 Post by mrsonchus » Tue Feb 10, 2015 9:25 pm

Manfred wrote:Dear all,
motivated by the section of a pine needle shown by Rod (rnabholz) I tried today the same.
Believe me - its a real challenge.
The section was stained with Wacker FCA.
Wow - superb results, congratulations these are excellent images!
Thanks for the post.

John
John B

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vasselle
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Re: Cross section of aneedle of Picea spec.

#6 Post by vasselle » Wed Feb 11, 2015 4:29 am

Bonjour
Très beau cliché
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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rnabholz
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Re: Cross section of aneedle of Picea spec.

#7 Post by rnabholz » Wed Feb 11, 2015 4:43 am

mrsonchus wrote:
rnabholz wrote:Perhaps it is my fresh experience, and less than optimal results speaking, but this is ABSOLUTELY FANTASTIC!!

I had planned to take another run at this subject tonight, but your shot may have defined the genre for all time.

Anything I might post will look like a mud pie now! ;^)

Would love to hear more about the technical details.

Rod
Hey you've made a great start yourself - I've not even produced anything that's not too bad to post and I've been
trying for a couple of months now. Your technique will definitely get better the more you practice.
I use images from others as my targets to achieve and am slowly making progress too.

Have another go, and then another - it's a great feeling when you see your own progress, especially when you
move into making permanently mounted slides for your own collection.

John
Thanks John. I am having too much fun to quit... ;^). See Pine Needle II for my second attempt.

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rnabholz
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Re: Cross section of aneedle of Picea spec.

#8 Post by rnabholz » Wed Feb 11, 2015 5:24 am

Manfred, (or anybody who would like to help)

What can you tell me about the Wacker FCA stain you used? I had not heard of it.

Thanks

Rod

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Re: Cross section of aneedle of Picea spec.

#9 Post by Manfred » Wed Feb 11, 2015 7:19 pm

Rod,

it is also named "Etzold FCA" and is a mixture of Fuchsin, Chrysoidin and Alcianblau (sorry but I don`t know in English).
It is usable for all plant sections and gives nice results.

Manfred

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mrsonchus
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Re: Cross section of aneedle of Picea spec.

#10 Post by mrsonchus » Thu Feb 12, 2015 1:29 am

Manfred wrote:Rod,

it is also named "Etzold FCA" and is a mixture of Fuchsin, Chrysoidin and Alcianblau (sorry but I don`t know in English).
It is usable for all plant sections and gives nice results.

Manfred
This stain certainly does a good job - I wonder what the 'UK version' is sold as?
Last edited by mrsonchus on Thu Feb 12, 2015 4:27 am, edited 1 time in total.
John B

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gekko
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Re: Cross section of aneedle of Picea spec.

#11 Post by gekko » Thu Feb 12, 2015 1:42 am

Manfred, this is absolutely superb (which is always the case with your photos). Gorgeous tapestry! Thank you for posting it.

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