My first feeble attempts to stain & mount onion epidermis...

[mrsonchus]

Here are several of my first rather poor attempts to stain onion epidermis
with Safranin and Fast Green stains (seperately).

The samples were kept for several days in 75% IPA then stained for 2 hours. They
were then taken through alcohol/DIW series down to DIW only, then mounted in
glycerin and sealed with nail-polish. A quick and dirty procedure I know but
I'm learning with every horror I produce!

Too much Safranin I think.......

websize_2_image_stitch.jpg (308.02 KiB) Viewed 3207 times

Onion epidermis nucleus

websize_onion_nucleus.jpg (204.52 KiB) Viewed 3207 times

Stoma pore on outer (lower) epidermis of onion leaf

web_size_stoma_2_image_stack.jpg (338.03 KiB) Viewed 3207 times

Higher magnification of stoma pore

web_size_weighted_average_stoma_2_image_stack.jpg (354.28 KiB) Viewed 3207 times

Sorry these are a bit messy-looking, my camera is not giving me anywhere near
the image quality that I see through my 'scope. I'm saving to buy a DSLR to mount
instead - hopefully a jump from 2mp Toupcam to an 18mp Canon will help with
photomicrograph quality?

Hope you like them

John

[gekko]

Hope you like them

I, for one, like them very much, especially 1 and 3. Very nice work!

[rnabholz]

Hey John,

They all look good from here. Nice job!

Rod

[vasselle]

Bonjour
Très belles séries.
J'aime beaucoup la dernière photo
Cordialement seb

[Crater Eddie]

Looks good from here! Makes me want to give it a go.
CE

[mrsonchus]

Looks good from here! Makes me want to give it a go.
CE

Thank you, Walter Dioni inspired me to try the onion epidermis, have a look at the website and his
contributions - you'll soon get bitten by the bug!

regards