GUF testing
GUF testing
After seeing the post Suphot made here I decided to give making a GUF a try. I spent the last few days re-reading over all posts in reference to this filter and printed several different designs, shapes, sizes and colour combinations to try at various stages of the light path.
I'm still experimenting to figure out the best location of the filter for my setup but so far I am really enjoying the results. I'm still on the fence if the oblique technique i use on my microscope yields better details but i wanted to quickly post the first images i snapped today. I didn't think to do a comparison of this object with the GUF and oblique...that would have made too much sense!!
Unfortunately my current sample dish doesn't have too much going on right now as all my water sources are frozen but i am hoping to get better samples later this week.
AO20, 10x/.30 PlanApo, 11 image stack
I'm still experimenting to figure out the best location of the filter for my setup but so far I am really enjoying the results. I'm still on the fence if the oblique technique i use on my microscope yields better details but i wanted to quickly post the first images i snapped today. I didn't think to do a comparison of this object with the GUF and oblique...that would have made too much sense!!
Unfortunately my current sample dish doesn't have too much going on right now as all my water sources are frozen but i am hoping to get better samples later this week.
AO20, 10x/.30 PlanApo, 11 image stack
- Attachments
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- QuickTest.jpg (163.61 KiB) Viewed 10586 times
Karl
AO21 with Canon M3
AO21 with Canon M3
Re: GUF testing
A pretty good start I'd say - there's definitely some 3D and textural detail there - well done.
John B
Re: GUF testing
Karl,
That image is beautiful. You could probably fool people onto believing that it is DIC.
Did you use blue gradient? The gentle gradient as shown in your image works really well at this magnification.
What I found in my experiments was that GUF works better (more contrast/less glare/more pleasing color)at lower than NA 0.65, for transparent protists. Above NA 0.65, offset oblique or COL works better, resolution wise. But it may depend on implementation (for example, my condensers may not offer the same access points for filters as yours).
That image is beautiful. You could probably fool people onto believing that it is DIC.
Did you use blue gradient? The gentle gradient as shown in your image works really well at this magnification.
What I found in my experiments was that GUF works better (more contrast/less glare/more pleasing color)at lower than NA 0.65, for transparent protists. Above NA 0.65, offset oblique or COL works better, resolution wise. But it may depend on implementation (for example, my condensers may not offer the same access points for filters as yours).
Re: GUF testing
I thought I was looking at DIC - really excellent results.
Keep going and be sure to educate us all on your process and tools.
Well done!
Rod
Keep going and be sure to educate us all on your process and tools.
Well done!
Rod
Re: GUF testing
Thanks John! I hope you are well! For $10 and about an hour of arts and crafts it'd be really neat to see what others can come up with!!
Fan - Thank you! I think i still have a ways to go before fooling the DIC experts but i'm getting closer! For this image yes, i used a blue gradient! I'm still not too sure which variation of the filter is giving me the best results but thank you for feedback! I remember reading about the NA "restrictions" with this type of filter so i will keep your experiences in mind. I'm wondering if theres a way to modify the filter for higher NA use. I'm having alot of fun experimenting though!
Rod - I appreciate the very kind comment! Thank you! Right now i have 3 different levels of gradient...in 2 different colours...in 3 different sizes...18 variations to play with! At some point this week once i get some better samples I'll post some comparisons of the same subject and see if theres a standout winner. I'm really looking forward to pushing this filter to its maximum potential and sharing the journey with everyone!
Fan - Thank you! I think i still have a ways to go before fooling the DIC experts but i'm getting closer! For this image yes, i used a blue gradient! I'm still not too sure which variation of the filter is giving me the best results but thank you for feedback! I remember reading about the NA "restrictions" with this type of filter so i will keep your experiences in mind. I'm wondering if theres a way to modify the filter for higher NA use. I'm having alot of fun experimenting though!
Rod - I appreciate the very kind comment! Thank you! Right now i have 3 different levels of gradient...in 2 different colours...in 3 different sizes...18 variations to play with! At some point this week once i get some better samples I'll post some comparisons of the same subject and see if theres a standout winner. I'm really looking forward to pushing this filter to its maximum potential and sharing the journey with everyone!
Karl
AO21 with Canon M3
AO21 with Canon M3
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Re: GUF testing
definitely great work, Karl. i am curious what portion of the frame you are capturing there? it's the # 1054, eyepiece?
Re: GUF testing
Very beautiful and interesting. It certainly seems made with DIC
Re: GUF testing
Beware such warnings...McConkey wrote:I remember reading about the NA "restrictions" with this type of filter so i will keep your experiences in mind.
"They didn't know it was impossible, so they did it." - Mark Twain
Can't wait to see more of your GUF images!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: GUF testing
Francisco - Thank you for the kind comment! It's pretty crazy how close this little filter replicates DIC!
Phil - The image was taken through the trinoc but no cat.#1054 eyepiece is being used. I never ended up getting one as i had to leave shortly after our discussion about it but now I'm back I'll start looking!! I'm still using the AO phototube with the already installed telon lens, the FoV is less than what you get via the eyepiece but it is parfocal, I've yet to figure out a way, with the accessories i have, to get full FoV via the trinoc AND have my camera parfocal. I'll keep my eyes open for the #1054!! Thanks for reminding me!
Kurt - ahah ok ok! I'll stop doubting myself! Thank you, I'm hoping to get some samples tomorrow or Thursday so the experiments can continue!!
Phil - The image was taken through the trinoc but no cat.#1054 eyepiece is being used. I never ended up getting one as i had to leave shortly after our discussion about it but now I'm back I'll start looking!! I'm still using the AO phototube with the already installed telon lens, the FoV is less than what you get via the eyepiece but it is parfocal, I've yet to figure out a way, with the accessories i have, to get full FoV via the trinoc AND have my camera parfocal. I'll keep my eyes open for the #1054!! Thanks for reminding me!
Kurt - ahah ok ok! I'll stop doubting myself! Thank you, I'm hoping to get some samples tomorrow or Thursday so the experiments can continue!!
Karl
AO21 with Canon M3
AO21 with Canon M3
Re: GUF testing
Bonjour
Très bon résultat car image est réussi avec un bon effet.
Cordialement seb
Très bon résultat car image est réussi avec un bon effet.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D
Boitier EOS 1200D + EOS 1100D
Re: GUF testing
Thanks Seb! I am really enjoying the effect of these filters!
Here is an image i managed to grab tonight with the GUF. The vorticella stayed still long enough for me to grab 7 frames. Unfortunately he was moving a little too much for me to do a full comparison of different GUF variations.
Taken with the black gradient, not quite as strong an effect as my original image but that's half the fun of experimenting!!
Here is an image i managed to grab tonight with the GUF. The vorticella stayed still long enough for me to grab 7 frames. Unfortunately he was moving a little too much for me to do a full comparison of different GUF variations.
Taken with the black gradient, not quite as strong an effect as my original image but that's half the fun of experimenting!!
- Attachments
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- AnotherTest.jpg (234.47 KiB) Viewed 10493 times
Karl
AO21 with Canon M3
AO21 with Canon M3
Re: GUF testing
Another test from today. I manged to collect some fresh water samples from a small creek not 100% frozen yet. While i'm waiting for it to settle i wanted to take a quick look and continue with the GUF testing. The mount has started to dry so it wasn't the best situation to start taking pictures but still fun regardless!
10 Image stack taken with the 10x /.30 Apo using the blue GUF. I'm thinking the blue combined with the 10x /.30 Apo is giving the best results.
10 Image stack taken with the 10x /.30 Apo using the blue GUF. I'm thinking the blue combined with the 10x /.30 Apo is giving the best results.
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- GUFtest3.jpg (453.69 KiB) Viewed 10457 times
Karl
AO21 with Canon M3
AO21 with Canon M3
Re: GUF testing
The Vorticella shot is nice enough, the oblique lighting and subdued BG really make it interesting. But that last image is wonderful! Bet you could fool more than a few into believing it's DIC. Super cool image!!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
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- Posts: 6327
- Joined: Fri May 15, 2015 12:15 am
Re: GUF testing
Microscope optics began their life using the blue of the sky as a light source and have evolved over time to utilize the neutralizing quality of blue light to soften and contrast the hard edged yellow to red wavelengths. For natural subjects it would be hard to see that some wavelength(s) in the blue section of the spectrum would not usually be best.
Re: GUF testing
For now i'm going to keep posting my GUF testing images here rather than flood the forum with additional topics!
Thank Kurt for the very kind words! This technique works the best at 10x and marginally alright at 20x. I think oblique is still better for 40x though as i havn't been able to recreate the effect as well past 20x.
Phil - Very....enlightening! See what i did there!! I'll keep the properties of blue in mind as i keep trying out different things with these filters! I will say that the blue is absolutely giving better results!
This image was taken using 20x /.50 AdvPlanAchro. Blue GUF filter placed under the condenser and position for best effect. Quick stack of 8 images, some stacking defects are noticeable but the little sucker was moving quite alot so i'm happy with what i managed to grab!
Thank Kurt for the very kind words! This technique works the best at 10x and marginally alright at 20x. I think oblique is still better for 40x though as i havn't been able to recreate the effect as well past 20x.
Phil - Very....enlightening! See what i did there!! I'll keep the properties of blue in mind as i keep trying out different things with these filters! I will say that the blue is absolutely giving better results!
This image was taken using 20x /.50 AdvPlanAchro. Blue GUF filter placed under the condenser and position for best effect. Quick stack of 8 images, some stacking defects are noticeable but the little sucker was moving quite alot so i'm happy with what i managed to grab!
- Attachments
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- GUFtest33.jpg (193.47 KiB) Viewed 10333 times
Karl
AO21 with Canon M3
AO21 with Canon M3
Re: GUF testing
I think this turned out real well..
BillT
BillT
Re: GUF testing
Wonderful work!
It's nobody's trouble but mine. I just want to make more for living, that's all. That's why I need to read 777spiel casino rules. Everything seems clear to me now.
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Re: GUF testing
Sorry to appear “thick”, but what is a guf filter? Can anyone post a link to what/how
Many thanks, John
Many thanks, John
Re: GUF testing
Thank you for pointing out that a bit of baseline info was omitted. The homemade filter in question is called a Gradient Universal Filter. I believe Wim van Egmond got the ball rolling on it, so Rod N. honored him by calling it the van Egmond filter. Here you go, these should get you going:
https://tinyurl.com/ybhsrxos
viewtopic.php?t=2686
https://tinyurl.com/ybhsrxos
viewtopic.php?t=2686
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
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- Joined: Sun Dec 14, 2014 5:20 pm
Re: GUF testing
Many thanks, Kurt. Guess what I will be doing this weekend. Best wishes, John
Re: GUF testing
Pictures, or it didn't happen.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: GUF testing
Hey John, the original post I made back in November had a link to the topic where the GUF is explained and all the details on how to make one. Here it is again for reference.
viewtopic.php?f=28&t=5449
One of the links Kurt posted is also linked in this link....ahah a link within a link within a link.... I'm dizzy already! Thanks for posting the links Kurt! The Van Egmond filters and GUF's are beyond incredible and really really effective! For $10 it's really impressive what you can achieve!!
There is also this topic which discusses the Van Egmond filter a little more.
viewtopic.php?f=14&p=33270#p33270
I can't wait to see your results and how you adapt it to your own scope! Also as Kurt said....Pics or it didn't happen! Really looking forward to seeing some images!!
Karl
viewtopic.php?f=28&t=5449
One of the links Kurt posted is also linked in this link....ahah a link within a link within a link.... I'm dizzy already! Thanks for posting the links Kurt! The Van Egmond filters and GUF's are beyond incredible and really really effective! For $10 it's really impressive what you can achieve!!
There is also this topic which discusses the Van Egmond filter a little more.
viewtopic.php?f=14&p=33270#p33270
I can't wait to see your results and how you adapt it to your own scope! Also as Kurt said....Pics or it didn't happen! Really looking forward to seeing some images!!
Karl
Karl
AO21 with Canon M3
AO21 with Canon M3
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- Posts: 48
- Joined: Sun Dec 14, 2014 5:20 pm
Re: GUF testing
Many thanks, Karl, I missed your original post. John
Re: GUF testing
No worries John! I blame the italics! I had a really fun time making these filters and experimenting with them, I hope they treat you just as well!! Feel free to ask any questions as you dive into the wonderful world of homemade filters!
*EDIT*....Thank you Bill and nickols for the kind words!
*EDIT*....Thank you Bill and nickols for the kind words!
Karl
AO21 with Canon M3
AO21 with Canon M3