Darkfield Sunflower stem

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mrsonchus
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Darkfield Sunflower stem

#1 Post by mrsonchus » Wed Dec 20, 2017 9:25 pm

Hi all, I've been slide-surfing and though I'd try to make darkfield with the x2.5 Leitz objective. I used a piece of Blu-Tack squashed into a diffusing-filter slipped into the filter tray beneath the condenser, top lens swung out.

Anyway it's interesting to see and I may well give it a try with a more accurately set stop, perhaps on live tissue too such as pollen grains.

Thought you may like to see the result, quite nice really - this was/is a stained and permanently-mounted slide of a Sunflower axial-branch stem in TS, sectioned at 10µ and mounted in Numount resinous mountant. The stains are Safranin and Fast-green, although of course this view is not the usual 'look'...
ws_darkfield-sunflower-stem.jpg
ws_darkfield-sunflower-stem.jpg (398.42 KiB) Viewed 2798 times
May try a few more.

John B. :)
John B

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Crater Eddie
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Re: Darkfield Sunflower stem

#2 Post by Crater Eddie » Wed Dec 20, 2017 9:49 pm

Very interesting... and somewhat haunting. More please!
CE
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JimT
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Re: Darkfield Sunflower stem

#3 Post by JimT » Wed Dec 20, 2017 10:13 pm

Very very good and yes, more. Really brings out the detail.

I have also done some dark field images of transverse sections (Not as good as this one) and based on your good results I will have to try some more.

Looks like that 2.5X obj. was a good acquisition.
Last edited by JimT on Wed Dec 20, 2017 10:57 pm, edited 1 time in total.

billbillt
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Re: Darkfield Sunflower stem

#4 Post by billbillt » Wed Dec 20, 2017 10:18 pm

I love this look!... Your usual perfect job and presentation...

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Re: Darkfield Sunflower stem

#5 Post by Hobbyst46 » Wed Dec 20, 2017 11:50 pm

Beautiful!
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billbillt
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Re: Darkfield Sunflower stem

#6 Post by billbillt » Thu Dec 21, 2017 12:00 am

.
Last edited by billbillt on Sat Dec 30, 2017 5:03 pm, edited 1 time in total.

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KurtM
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Re: Darkfield Sunflower stem

#7 Post by KurtM » Thu Dec 21, 2017 1:38 am

That's a really pretty image, looks like an entry in one of those contests to me! 8-)
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mrsonchus
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Re: Darkfield Sunflower stem

#8 Post by mrsonchus » Thu Dec 21, 2017 3:48 pm

Crater Eddie wrote:Very interesting... and somewhat haunting. More please!
CE
Thanks Ed' - yes, if I glance sideways at it it reminds me of one of those 'big round space-entities' coming towards the Starship Enterprise - the original of course! :D :D
John B

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mrsonchus
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Re: Darkfield Sunflower stem

#9 Post by mrsonchus » Thu Dec 21, 2017 3:52 pm

JimT wrote:Very very good and yes, more. Really brings out the detail.

I have also done some dark field images of transverse sections (Not as good as this one) and based on your good results I will have to try some more.

Looks like that 2.5X obj. was a good acquisition.
Hi Jim, good to hear from you!

Yes, I was really surprised that a decent image came out - quite pleased! :D

The two major factors seem to be the edge of the stop is in focus with the specimen, and that the stop is the right size of course.
The 100W bulb I have in the Orthoplan at the moment easily gave me the high light-level needed to get the image this bright with the field jet-black.

Yes, I'm very pleased with the x2.5 indeed! Thanks Jim. :D :D :)
John B

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mrsonchus
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Re: Darkfield Sunflower stem

#10 Post by mrsonchus » Thu Dec 21, 2017 3:53 pm

billbillt wrote:I love this look!... Your usual perfect job and presentation...

BillT
Hi Bill - thanks my friend - yes I found the look really pleasing too - seems so finely detailed. :D :D

p.s. I like the look of the new objective - get it in the turret! I'd love to see how it perform for you. :D :D
John B

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mrsonchus
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Re: Darkfield Sunflower stem

#11 Post by mrsonchus » Thu Dec 21, 2017 3:56 pm

KurtM wrote:That's a really pretty image, looks like an entry in one of those contests to me! 8-)
Haha! Thanks Kurt, yes it's quite an eye-candy affair this darkfield stuff - great tissue delineation between the epidermal, the cortical, vascular and ground systems - really clearly visible in the overview. Must try some more of this technique perhaps with some better cut-out stops.

Thanks Kurt. :D :D :)
John B

charlie g
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Re: Darkfield Sunflower stem

#12 Post by charlie g » Thu Dec 21, 2017 6:32 pm

Beautiful colors, Mr.Sonchus. I love the blue coloration, like a stained glass window to my eyes. Terrific anatomy on a dark winters night! Charlie guevara

einman
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Re: Darkfield Sunflower stem

#13 Post by einman » Sat Dec 30, 2017 4:06 am

Very nice indeed

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vasselle
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Re: Darkfield Sunflower stem

#14 Post by vasselle » Thu Mar 01, 2018 9:02 pm

Bonjour
Superbe image
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mrsonchus
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Re: Darkfield Sunflower stem

#15 Post by mrsonchus » Thu Mar 01, 2018 9:54 pm

vasselle wrote:Bonjour
Superbe image
Thanks Seb - pleased you like it. :D
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Re: Darkfield Sunflower stem

#16 Post by apochronaut » Thu Mar 01, 2018 10:56 pm

I can't remember, John; if you have phase on your Leitz? If you have, the phase annulus for the 100X objective, makes a good DF stop for low power objectives, 10X and under. The 40X annulus can be used for very low power objectives. They are nicely centerable too.

nice image.

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Re: Darkfield Sunflower stem

#17 Post by photomicro » Thu Mar 01, 2018 11:05 pm

apochronaut wrote:I can't remember, John; if you have phase on your Leitz? If you have, the phase annulus for the 100X objective, makes a good DF stop for low power objectives, 10X and under. The 40X annulus can be used for very low power objectives. They are nicely centerable too.

nice image.
Great minds think alike eh John? Quite ironic.

Sometimes a similar (but different!) effect can be achieved with polars.
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JimT
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Re: Darkfield Sunflower stem

#18 Post by JimT » Thu Mar 01, 2018 11:12 pm

Sometimes a similar (but different!) effect can be achieved with polars.
Ah yes. Plant sections in polarized light can be enjoyable as well as very revealing of hidden details.

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billben74
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Re: Darkfield Sunflower stem

#19 Post by billben74 » Thu Mar 01, 2018 11:17 pm

I like it. A lot.
Fabulous image. Now where is my dark filed condenser.....

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mrsonchus
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Re: Darkfield Sunflower stem

#20 Post by mrsonchus » Fri Mar 02, 2018 12:10 am

Hi, yes the polariser is great for lignin, the xylem secondary lignified cell walls glow!

Here are a couple of examples of polarised xylem lignin,

Compared to 'normal' brightfield,
ws_brighfield_polarized.jpg
ws_brighfield_polarized.jpg (161.65 KiB) Viewed 2443 times
Looking down into a vessel with polariser,
ws_xylem_polarized_birefringence.jpg
ws_xylem_polarized_birefringence.jpg (30.17 KiB) Viewed 2443 times
Should be able to get the big-O up & running soon with the new 402a rig!
I'll post a heap of images as I put it through it's paces.
Of course the 402a alsohas a darkfield stop which should be interesting to try on perhaps some moss parts and pollen grains....

Back soon...
John B

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mrsonchus
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Re: Darkfield Sunflower stem

#21 Post by mrsonchus » Fri Mar 02, 2018 12:14 am

apochronaut wrote:I can't remember, John; if you have phase on your Leitz? If you have, the phase annulus for the 100X objective, makes a good DF stop for low power objectives, 10X and under. The 40X annulus can be used for very low power objectives. They are nicely centerable too.

nice image.
I just bought a 402a Apo' - and it's just arrived, as have x10, x25 and x40 Phaco objectives to go with it - just waiting for the adjuster wrenches to arrive although I'm improvising a little with a pair of fine forceps to a reasonable extent as a starter...

Thanks Apo' :D :D
John B

charlie g
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Re: Darkfield Sunflower stem

#22 Post by charlie g » Fri Mar 02, 2018 2:49 am

Your first df image stunning, Mr.Sonchus..like a peek at Louvre, France stained glass windows. thanks for these df contrast method colors. Charlie guevara

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