Onion stoma using Canon EOS 1200D

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
mrsonchus
Posts: 4175
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Onion stoma using Canon EOS 1200D

#1 Post by mrsonchus » Sat Feb 14, 2015 9:52 pm

Hi, I've just taken this 6-picture stack (stacked with Toupview) of an onion epidermis stoma through
a 60x objective...
Taken with Canon EOS 1200D
Taken with Canon EOS 1200D
websize_tv_maxc_6stack.jpg (352.52 KiB) Viewed 3108 times
I'm pretty pleased with the level of detail, I like the nucleus detail with it's nucleoli bottom left too. The Canon is definitely a big step up from the 2mp Toupcam.

Here's a 4-image stack through the 40x objective
Seems pretty sharp - progress!
Seems pretty sharp - progress!
websize_tv_maxc_4stack.jpg (423.5 KiB) Viewed 3108 times
Schlembergera with pollen-tube starting to emerge from pollen grain.
Mounted in Fuschin Glycerin Jelly
Mounted in Fuschin Glycerin Jelly
websize_schlembergera_pollen_tube_emerging.jpg (293.93 KiB) Viewed 3108 times
Sonchus pollen without any background cleanup....
Better without processed background....
Better without processed background....
websize_sonchus_asper_pollen.jpg (296.89 KiB) Viewed 3108 times
Hope you like them.
John B

User avatar
vasselle
Posts: 2763
Joined: Sat Oct 25, 2014 5:32 pm
Location: France

Re: Onion stoma using Canon EOS 1200D

#2 Post by vasselle » Sun Feb 15, 2015 6:10 am

Bonjour
Très belles séries images.
J'aime particulièrement celle que vous avais fait sur les pollens elles sont belles.
Très bon travail,merci pour le partage.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

User avatar
mrsonchus
Posts: 4175
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Onion stoma using Canon EOS 1200D

#3 Post by mrsonchus » Sun Feb 15, 2015 11:09 am

vasselle wrote:Bonjour
Très belles séries images.
J'aime particulièrement celle que vous avais fait sur les pollens elles sont belles.
Très bon travail,merci pour le partage.
Cordialement seb
Thank you very much vasselle for your kind comments
John B

User avatar
75RR
Posts: 8207
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Onion stoma using Canon EOS 1200D

#4 Post by 75RR » Sun Feb 15, 2015 11:15 am

Taken with Canon EOS 1200D
Congratulations on the new camera. How have you attached it?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

JimT
Posts: 3247
Joined: Fri Oct 24, 2014 1:57 pm

Re: Onion stoma using Canon EOS 1200D

#5 Post by JimT » Mon Feb 16, 2015 4:26 am

The 60x onion image is good but I see more detail in the 40X. Too much enlargement???
Nice camera. How have you mounted it?

User avatar
mrsonchus
Posts: 4175
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Onion stoma using Canon EOS 1200D

#6 Post by mrsonchus » Mon Feb 16, 2015 6:18 am

JimT wrote:The 60x onion image is good but I see more detail in the 40X. Too much enlargement???
Nice camera. How have you mounted it?
I agree, but the 60x is very sensitive to mount quality and the proximity of specimen to underside of coverglass, also the refractive index of the mounting medium and the overall thickness and flatness or the slide..... (I've just been reading about the importance of coverglass characteristics as objective power and NA rises, by Mr Kim)...
I also think my choices of focal planes for the constituent pictures of the stacks was definitely sub-optimal!
Basically I made a right old mess of it and didn't do the 60x any justice at all - through the 'scope it's a real performer.
I'll try another demo with some better slides and an improved technique (I hope..).

I'm going to post the Camera to 'scope mount details as soon as I've got some pictures of the parts together for the post.

regards
John B

Post Reply