Yet more from the Haematococcus culture jar

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Crater Eddie
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Yet more from the Haematococcus culture jar

#1 Post by Crater Eddie » Thu Feb 19, 2015 5:00 am

Every time I look at a sample from the Haemotococcus culture jar I see things that I just have to take photos of. I'm not sure what the colorless blobs intermingled with the Haematococcus here are.
These are all taken with the stock 40x objective.


Image




I almost always see one or more of these spiky fellows. They don't zip around like ciliates, they glide very slowly.

Image




I have never seen this one (center) before.

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Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
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gekko
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Re: Yet more from the Haematococcus culture jar

#2 Post by gekko » Thu Feb 19, 2015 11:53 am

Wow! You are really successful in Haematococcus farming! Wonderful. Nice images too. I don't know what the spiky fellow is, but the green desmid in the middle of the last photo may be Cosmarium.

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rnabholz
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Re: Yet more from the Haematococcus culture jar

#3 Post by rnabholz » Thu Feb 19, 2015 1:57 pm

That red is just crazy! Very cool images and critters.

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Crater Eddie
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Re: Yet more from the Haematococcus culture jar

#4 Post by Crater Eddie » Thu Feb 19, 2015 2:56 pm

Thanks for the comments!

This culture bottle is a constant wonder to me. It never ceases to provide hours of entertainment whenever I take a sample. I have noticed that the critter population is shifting ever so slowly. At first there was almost no green to be seen in there anywhere, now it is slowly on the rise. I keep saying that I need to start a new bottle before the population in this one collapses, I think it's time.

I was struggling with the focus on these photos... I recently traded out my very old crt monitor for a newer but still old flat screen monitor figuring it would be more clear and crisp, in addition to taking up much less room on the desk. Bad move! While it is much more compact, the screen resolution is poor compared to the crt making critical focusing difficult. I need to upgrade my work room computer. I'm still running Windows XP. :roll:
CE
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
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vasselle
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Re: Yet more from the Haematococcus culture jar

#5 Post by vasselle » Fri Feb 20, 2015 2:09 pm

Bonjour
Très belles séries images
Cordialement seb
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charlie g
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Re: Yet more from the Haematococcus culture jar

#6 Post by charlie g » Sat Feb 21, 2015 6:54 pm

The red pigment is a very good antioxident nutrient which your hardy protist: Haematococcus pluvialis makes. Everything including pink flamingo birds, pink fleshy salmon fish, pink fleshy shrimp..and we humans ingest this protist made pigment! They have huge open air culture tanks in Hawaii (I think in Hawii)..where they commercially grow/culture your Haematoccus, Crater Eddie. The pigment is called astazanthine.

Your protists use it as an internal 'sun screen protection'. They can move the location of this pgment around in their cytoplasm...often to just shield their nucleus fron sunlight, so in this configuration you observe the granny-apple green flagelate...with the apple red central region..lovely colors.

I have a terra cotta bird bath in a back garden where this hardy protist took up residence a few years ago. The bird bath shattered from ice fracture before I got to drain that bath...I saved a few chunks of that bath,,the bright red resting phase will emerge when in spring I put up a new bird bath.

It's good to see your cultures are holding up...after a while..I sort of convert so much of my home into balanced aquatic communities...with a minds eye to microscopy.

One day, Eddie, set up a wynegradskie (?sp?) flask...I have one on my microscope bench which has been going for years...a tiny balanced wetlands, with specific habitats where microscopy is waiting for all. all the best, charlie guevara

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75RR
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Re: Yet more from the Haematococcus culture jar

#7 Post by 75RR » Sat Feb 21, 2015 11:32 pm

Great brew you got going there Eddie!

Link to Sergei Winogradsky poster: Download link is on the right of the page. Fun poster.

http://www.hhmi.org/biointeractive/post ... ion-bottle
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Crater Eddie
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Re: Yet more from the Haematococcus culture jar

#8 Post by Crater Eddie » Sun Feb 22, 2015 4:14 am

Thanks for the link! In addition to the poster, there are also instructions for making Winogradsky columns. Very interesting, and some good ideas there.
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)

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Crater Eddie
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Re: Yet more from the Haematococcus culture jar

#9 Post by Crater Eddie » Mon Feb 23, 2015 7:16 pm

Thanks for that information Charlie. I am curious about your flask... do you ever have to add any sort of nutrients to it, or manage it in any other way?
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)

einman
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Re: Yet more from the Haematococcus culture jar

#10 Post by einman » Tue Feb 24, 2015 2:13 am

The spiky creature could be a Heliozoon. Difficult to tell what genus. So I will throw several out to start conversation:

Heterophyrs
possibly Acanthocystis or Oxnerella.

With some closer observation we could possibly key it to genus.

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Crater Eddie
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Re: Yet more from the Haematococcus culture jar

#11 Post by Crater Eddie » Tue Feb 24, 2015 2:30 am

Hmmm... you could be right. He is radially symmetrical, mostly. I see them quite often in samples from this bottle, I'll try to get some more photos.
Olympus BH-2 / BHTU with Olympus E-P1 MFT camera mounted
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)

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