MicrobeHunter.com Microscopy Forum

Hello,
like to share a foto from Paramecium bursaria, nothing new but hope you like it.



Sincerely
Jochen

Beautiful image! Thanks for posting!

Impressive!

Great image!

Very crisp and clear image.. Thanks for sharing....

BillT

Ditto the above - superbly clear, crisp image - nicely done!

John B.

Great capture Rex, which of those microscopes did you take this one on, the Standard RA.

Glad that you like the pic.
Its from the vertical Zeiss IM35 microscope which has flash and DIC equipment. I use large cover glasses as carrier, so it has the right thickness of 0.17 and I can use the convential objectives & not the large distance ones for dishes.

As we all have cheered, Imperatorrex, bravo protest image, thanks for posting to share.

With your 'flash illumination'...you had no need for 'image stacking'? Was it difficult to keep this delightful ciliate in a crisp focus for it's cilia borders? Was this paramecium fast moving at time of this very sharp focus of it's cilia border?

Or did you somehow use a collection of stacked images'?

Beautiful image of a protozoan with it's symbiotic algae internal garden, again, bravo to our green enriched planet..you have it here in your image capture, Imperatorrex. Charlie guevara

Hi Charlie,
the picture is not a stack. The flash is really the key - the extremly short flash time itself somehow "freezes" the cilliate movement. Also the optical section by DIC really helps to make it sharp and crispy.

I am curious how it is with a moving protozoan...how do you know what optical section has the best image capture of the cilia, and a large number of those endosymbiont algae cells? I thought your 'depth of crisp focus' would be quite shallow, quite brief on moving organisms..for your then 'freeze action flash'..doesn't the 'freeze action flash' also image capture a slightly...or greatly out of focus target organism?

When do you sense your 'in a good optical section plane' and hit your flash/image capture 'button'? Or is it hit and miss with a series of optical sections captured..you share the best one?

Again, terrific image of one of my favorite protists! I'd love to transfer images such as yours to: ceramic wall tiles, or to wall paper for my study! Charlie guevara

If the protists are moving fast it is certainly just a lot of hit and miss. But the paramecium is not such a difficult one. I am sure he has rested from time to time. Especially when there are some algaes or detrius is present.

Another technique is to wait until the ciliate is just captured by the cover glass pressure, when the water layer becomes thiner after some of the water has vaporized.
However I am sure that the vertical microscope like my IM35 also helps, stuff like the algae or the protists seem to rest on the buttom by gravity?