Floscularia melicerta

[ImperatorRex]

Floscularia melicerta - a photo I took some years ago:

(thumbnail, click to view larger image):


If you are interested I also have two short videos (~ 1min] to share

Video taken with a Plan 6,3 DIC, gives a kind of overview:


Video taken with 25x Objective - much more details:


Hope you like it.

[75RR]

Great image and videos - particularly the 25x
What version of DIC did you use with it?

[ImperatorRex]

Muchas gracias 75RR.
Videos were taken with the "newer" Zeiss finite DIC.

[75RR]

Videos were taken with the "newer" Zeiss finite DIC

Plan Neofluar 25x/0.8 W Oel Glyc .......? So it is as good as I imagined.
Putting it on my wish list ... now all I need is a fairy godmother who is willing to lend a hand :)

[ImperatorRex]

Plan Neofluar 25x/0.8 W Oel Glyc .......? So it is as good as I imagined.

Actually video was taken with my favorite objective, the Zeiss Planapo 25/0,65. This objective provides a quite nice image. It is also because of convenience, it is a dry obejctive but also provides an adequate working distance (even the Zeiss catalogue states it is only 0,12 mm...my impression is, it offers more).

No question the Multi Immersion 25/0,80 is great, but it has his price (guess you will pay 3 times compared to the Planapo). Working distance it quite limited (only 0,09 mm), and finally the immersion always is less convenient than the dry objective.

So the Planapo 25/0,65 usually keeps me happy

[75RR]

Actually video was taken with my favorite objective, the Zeiss Planapo 25/0,65. This objective provides a quite nice image. It is also because of convenience, it is a dry obejctive but also provides an adequate working distance (even the Zeiss catalogue states it is only 0,12 mm...my impression is, it offers more).

Good point on the working distance. So what slider did you use?

[ImperatorRex]

Hi 75RR,

So what slider did you use?

Have a look here, addes some pics to explain:
viewtopic.php?f=24&t=5930&p=53567#p53567

[75RR]

I think we may be talking at cross purposes here.

There are 4 Zeiss DIC systems for the 160 tube microscopes.

See link: viewtopic.php?f=15&t=1543

I was under the impression that you had used the 4th and latest of the DIC 160 systems in the video.

It seems not :)
I just assumed it was a DIC video - hence my questions.

[ImperatorRex]

I was under the impression that you had used the 4th and latest of the DIC 160 systems in the video.

Yes it is exacetly this latest DIC system that I have used in the video. The DIC manual documents the Zeiss tested and recommended Objective / Wollaston / DIC slider combinations. However sometimes different combinations will also work for objectives, where Zeiss did not have made specific DIC slides.

For the PlanApo 25 I found that the DIC slider for the Plan 6,3 Obektive works very well (the video was made with this DIC slider). The DIC slider for Plan Neofluar 25/0,8 works even a bit better - it produceses less gradient.

Beside the various DIC sliders, there are 4 different Wollaston prisms and 3 different front lenses (0,63; 0,9; 1,4 nA) that can combine. So lots combinations to test! I think I have tried more than 200 out of them.

[vasselle]

Bonjour
Très belle vidéo

[ImperatorRex]

Bonjour Jolie, je suis content que tu aimes la vidéo.