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another Diatom

Posted: Thu Apr 12, 2018 8:45 pm
by ImperatorRex
A diatom in vivo. The photo is not stacked.

Image

Re: another Diatom

Posted: Thu Apr 12, 2018 9:01 pm
by JimT
Fantastic!

Re: another Diatom

Posted: Thu Apr 12, 2018 9:06 pm
by Hobbyst46
Stunning!
Some technical details would be most welcomed: What size is the diatom ? which optics was used for imaging? Thanks in advance.

Re: another Diatom

Posted: Thu Apr 12, 2018 9:12 pm
by 75RR
Beautiful image. A surprisingly large area in focus too for a single image.

Re: another Diatom

Posted: Thu Apr 12, 2018 10:10 pm
by billbillt
THAT IS AMAZING QUALITY FOR AN UNSTACKED IMAGE..........

BILLT

Re: another Diatom

Posted: Fri Apr 13, 2018 1:13 pm
by vasselle
Bonjour
Very nice image

Re: another Diatom

Posted: Sun Apr 15, 2018 1:58 am
by Radazz
WOW!

Re: another Diatom

Posted: Sun Apr 15, 2018 8:22 am
by ImperatorRex
Thank you for the feedback, it motivates to post more :-)
Hobbyst46 wrote:Stunning!
Some technical details would be most welcomed: What size is the diatom ? which optics was used for imaging? Thanks in advance.
Size is about 200 um. Photo was taken with the Zeiss IM35 (vertical) microscope. It uses the old Zeiss finite DIC, objective was the Planapo 25/0,65.
75RR wrote:Beautiful image. A surprisingly large area in focus too for a single image.
Maybe it is because the focus on the raphne of the diatom and with a vertical microscope this area rests directly on the cover glass?

Anybody who can help to identify this diatom?

Re: another Diatom

Posted: Sun Apr 15, 2018 9:08 am
by 75RR
Maybe it is because the focus on the raphne of the diatom and with a vertical microscope this area rests directly on the cover glass?
Could well be. As you mentioned earlier - gravity is your friend if you have an inverted microscope :)

Re: another Diatom

Posted: Wed Apr 18, 2018 4:40 pm
by ImperatorRex
Same diatom but with Plan 16 / DIC:

Image

Re: another Diatom

Posted: Sun Apr 22, 2018 6:23 am
by hkv
Really nice work! Details and lightning are stunning!

Re: another Diatom

Posted: Mon Apr 23, 2018 7:37 pm
by ImperatorRex
Thank you HÃ¥kan for your feedback. By the way: I just had a first look on some of you photos that you share on flickr. They are very very impressive! I will need much more time to have a closer look. They are probabely the result of your Olympus BX51?