Shamrock pollen through different mag. eyepieces.

[The QCC]

My current microscope setups are limited to 40x objectives and 2.5x photo eyepieces and a maximum magnification of 100x. This is fine for thin sections, but a bit lacking for biological specimens.
I do have the original 70 year old Bausch and Lomb 5x, 10x and 15x eyepieces.
These old eyepieces would give me approximately 200x, 400x and 600x.

The linked photos show Shamrock pollen through the three original B & L eyepieces with one photo using the Nikon PL 2.5x projection lens.
I apologize for the dirty images. The eyepieces are old and have not been used in decades and I did nothing more than a quick dusting with a micro fibre cloth.

Click on the image to see the linked files. Use the left <- and -> right arrow keys to view the photos.

ShamrockPollen_XSNB_40x_211_PDS_T.jpg (75.92 KiB) Viewed 2923 times

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[JimT]

Those really are good. How big (small) is the pollen?

[mrsonchus]

Beautiful images, great texture from the stacking. What version of combineZP are you using and how do you find it in general - these results look good enough to justify buying it....

[The QCC]

This is image 1 of 11 with measurements.
Total magnification is 100x. 40x obj. * 2.5x NIikon PL.
About CombineZP. It is free and the last version is available from CombineZP download
I run the All macros routine and then save the best of the six methods.

ShamrockPollen_XSNB_40x_201.jpg (98.44 KiB) Viewed 2909 times

The measurement software is called DigCam from Science 4 All.
It is donation ware and works quite well.

[mrsonchus]

About CombineZP. It is free and the last version is available from CombineZP download
I run the All macros routine and then save the best of the six methods.

The measurement software is called DigCam from Science 4 All.
It is donation ware and works quite well.

That's great - got them both now - I'm going to need a calibration slide but I'll start with the good old 1mm div ruler for the x4! Many thanks for this info.

[vasselle]

Bonjour
Très belle photo de pollen
Cordialement seb