Haematococcus pluvialis

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ImperatorRex
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Haematococcus pluvialis

#1 Post by ImperatorRex » Mon Apr 30, 2018 2:22 pm

Haematococcus pluvialis has various "faces". I wonder what stages you have observerd. Pls. feel free to share! Does anybody have seen stages "c" and "h" - see fig. 1 below?

The development cycle of Haematococcus pluvialis is represented as followed (according to ELLIOT, see also footnote 1):

Fig. 1: Palm cell states (a) can divide into either flagellated cells (h) or again palmellacellas, and the resulting cellular cell states (g) grow into" adult "forms (a) and the circulation can begin anew, but if flagellated Cells form, the cycle continues with the formation of young macrozoospores (f), which soon become normal macrozoospores (e). Periodically, these macrozoospores lose their flagella and turn into a palmella-like form (a), although their From this condition, young macrozoospores (f) are formed and the circulation is repeated to infinity.Hematocysts (b) can be formed from macrozoospores (e), although the usual process leads to the formation of palm cells (a). Under certain conditions, microzoospores (c) are formed from red cysts, which soon turn into young palmella-like states (d), which develop into either M acrozoospores (e) or become Palmella states (a). In both cases the circulation starts from here in the usual way. According to ELLIOT.
Image

Below some photos I made in 2016:

Thick-walled, unspotted and red-colored Aplanospore. The red, cytoplasmic carotenoid astaxanthin overlays existing structures. Depending on the focus, the core could also be seen in the Aplanospore shown here (center photo / right). Maybe the state of development correspont to "(d) - young palmella-like state"?
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Green, flagellated form, probably "macrozoospores". In addition, intermediate stages with more or less pronounced mucous envelopes.
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F Flagellum, G Mucous shell , P Pyrenoid, PF Plasma Precursors, N Nuclei / Core, S Stigma (Eye Patch)
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The flagella of hematococcus shall pass through "tubes" through the outer sheath (Huber-Pestalozzi "Volvococcales"). I think they are difficult to observe. Does anybody spotted and documented this tubes? I will try later on my side to find ... I have a pot with red water in the garden ;-)

More photos, these show different palmella-like states. The colors vary a lot, ranging from green, yellow, red to brown. I can hardly resist to make too many photos, it is always so existing observing. I try to restrict to show only a few here ;-)

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Print Media:
(1) Müller, Justus, Kulturversuche mit Haematococcus pluvialis, Mikrokosmos 43, 225 http://www.zobodat.at/pdf/Mikrokosmos_43_0001.pdf
(2) Aumann, G, Haematococcus pluvialis und das Bamberger Blutbecken. Mikrokosmos 43, 99 http://www.zobodat.at/pdf/Mikrokosmos_43_0001.pdf

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75RR
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Re: Haematococcus pluvialis

#2 Post by 75RR » Mon Apr 30, 2018 2:56 pm

Many thanks for the lesson. Enjoyed it! :) :)
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Re: Haematococcus pluvialis

#3 Post by SunshineLW » Mon Apr 30, 2018 3:46 pm

Incredible images! Thank you for sharing!

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Re: Haematococcus pluvialis

#4 Post by billbillt » Mon Apr 30, 2018 3:51 pm

this is a very interesting sequence that you have presented here... Thanks!..

BillT

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Re: Haematococcus pluvialis

#5 Post by Crater Eddie » Mon Apr 30, 2018 8:25 pm

Superb images and great information! We had a lot of discussion and images of haemotococcus several years ago, but not so much the last few years. Perhaps your post here will renew interest.
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Re: Haematococcus pluvialis

#6 Post by desertrat » Mon Apr 30, 2018 8:27 pm

The clarity and detail is amazing!
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Re: Haematococcus pluvialis

#7 Post by ImperatorRex » Wed May 02, 2018 6:33 pm

Thank you all for the encouraging feedback.

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Re: Haematococcus pluvialis

#8 Post by vasselle » Sat May 19, 2018 1:09 am

Bonjour
Superbe travail avec de magnifiques images d'un très grande clarté.
Merci pour le partage
Cordialement seb
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Re: Haematococcus pluvialis

#9 Post by JimT » Sat May 19, 2018 11:20 pm

First off great images and thanks for the tutorial. I think I have seen some in their green state.
We had a lot of discussion and images of haemotococcus several years ago
Geko, who was active here until about two years ago use to post many nice images of red Heamatococcus.

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Re: Haematococcus pluvialis

#10 Post by 75RR » Sun May 20, 2018 6:30 am

Geko, who was active here until about two years ago use to post many nice images of red Heamatococcus.
I remember them well. I believe it was Crater Eddie that provided him with a very productive sample.
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Re: Haematococcus pluvialis

#11 Post by EarleGar » Wed May 23, 2018 1:22 pm

Absolutely stunning images. Some of those look like art, and they really are, quite frankly.

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Re: Haematococcus pluvialis

#12 Post by ImperatorRex » Thu May 24, 2018 10:16 pm

Thanks all for the feedback.

Acc. to literature the flagella of hematococcus shall pass through "tubes" through the outer sheath. It is difficult to identify any details on photos.

I spent some time to get details, here is what I was able to capture on photos:

Tube broken by cover glass pressure, flagellum splitted outside the tube
Image

Under unfavorable conditions, motile cells lose their flagella and transform into non-motile cells. Think the photo below shows such, flagellata is already lost and the empty tubes (Tb) remain inside the sporangium:

Image

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Re: Haematococcus pluvialis

#13 Post by Radazz » Fri May 25, 2018 6:44 pm

I found this in my birdbath this morning.
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ImperatorRex
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Re: Haematococcus pluvialis

#14 Post by ImperatorRex » Fri May 25, 2018 7:28 pm

Yes, Radazz. Look like you got the Haematococcus pluvialis spores. Lets see if you also get the motive macrozoospores.

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Re: Haematococcus pluvialis

#15 Post by hkv » Thu May 31, 2018 10:48 pm

Fantastic images! Thanks for sharing! I need to look for these!
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Re: Haematococcus pluvialis

#16 Post by MicrobeGazer45 » Fri Apr 17, 2020 10:33 am

Greta images! Very useful.
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