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Haematococcus pluvialis has various "faces". I wonder what stages you have observerd. Pls. feel free to share! Does anybody have seen stages "c" and "h" - see fig. 1 below?

The development cycle of Haematococcus pluvialis is represented as followed (according to ELLIOT, see also footnote 1):

Fig. 1: Palm cell states (a) can divide into either flagellated cells (h) or again palmellacellas, and the resulting cellular cell states (g) grow into" adult "forms (a) and the circulation can begin anew, but if flagellated Cells form, the cycle continues with the formation of young macrozoospores (f), which soon become normal macrozoospores (e). Periodically, these macrozoospores lose their flagella and turn into a palmella-like form (a), although their From this condition, young macrozoospores (f) are formed and the circulation is repeated to infinity.Hematocysts (b) can be formed from macrozoospores (e), although the usual process leads to the formation of palm cells (a). Under certain conditions, microzoospores (c) are formed from red cysts, which soon turn into young palmella-like states (d), which develop into either M acrozoospores (e) or become Palmella states (a). In both cases the circulation starts from here in the usual way. According to ELLIOT.


Below some photos I made in 2016:

Thick-walled, unspotted and red-colored Aplanospore. The red, cytoplasmic carotenoid astaxanthin overlays existing structures. Depending on the focus, the core could also be seen in the Aplanospore shown here (center photo / right). Maybe the state of development correspont to "(d) - young palmella-like state"?


Green, flagellated form, probably "macrozoospores". In addition, intermediate stages with more or less pronounced mucous envelopes.



F Flagellum, G Mucous shell , P Pyrenoid, PF Plasma Precursors, N Nuclei / Core, S Stigma (Eye Patch)


The flagella of hematococcus shall pass through "tubes" through the outer sheath (Huber-Pestalozzi "Volvococcales"). I think they are difficult to observe. Does anybody spotted and documented this tubes? I will try later on my side to find ... I have a pot with red water in the garden

More photos, these show different palmella-like states. The colors vary a lot, ranging from green, yellow, red to brown. I can hardly resist to make too many photos, it is always so existing observing. I try to restrict to show only a few here













Print Media:
(1) Müller, Justus, Kulturversuche mit Haematococcus pluvialis, Mikrokosmos 43, 225 http://www.zobodat.at/pdf/Mikrokosmos_43_0001.pdf
(2) Aumann, G, Haematococcus pluvialis und das Bamberger Blutbecken. Mikrokosmos 43, 99 http://www.zobodat.at/pdf/Mikrokosmos_43_0001.pdf

Many thanks for the lesson. Enjoyed it! :) :)

Incredible images! Thank you for sharing!

this is a very interesting sequence that you have presented here... Thanks!..

BillT

Superb images and great information! We had a lot of discussion and images of haemotococcus several years ago, but not so much the last few years. Perhaps your post here will renew interest.
CE

The clarity and detail is amazing!

Thank you all for the encouraging feedback.

Bonjour
Superbe travail avec de magnifiques images d'un très grande clarté.
Merci pour le partage
Cordialement seb

First off great images and thanks for the tutorial. I think I have seen some in their green state.

We had a lot of discussion and images of haemotococcus several years ago

Geko, who was active here until about two years ago use to post many nice images of red Heamatococcus.

Geko, who was active here until about two years ago use to post many nice images of red Heamatococcus.

I remember them well. I believe it was Crater Eddie that provided him with a very productive sample.

75RR wrote: ↑

Sun May 20, 2018 6:30 am

Geko, who turned to Bluehost and was active here until about two years ago use to post many nice images of red Heamatococcus.

I remember them well. I believe it was Crater Eddie that provided him with a very productive sample.

Absolutely stunning images. Some of those look like art, and they really are, quite frankly.

Thanks all for the feedback.

Acc. to literature the flagella of hematococcus shall pass through "tubes" through the outer sheath. It is difficult to identify any details on photos.

I spent some time to get details, here is what I was able to capture on photos:

Tube broken by cover glass pressure, flagellum splitted outside the tube


Under unfavorable conditions, motile cells lose their flagella and transform into non-motile cells. Think the photo below shows such, flagellata is already lost and the empty tubes (Tb) remain inside the sporangium:

I found this in my birdbath this morning.
12 shot stack
Zeiss Axioskop. 40x ph2.

Yes, Radazz. Look like you got the Haematococcus pluvialis spores. Lets see if you also get the motive macrozoospores.

Fantastic images! Thanks for sharing! I need to look for these!

Greta images! Very useful.