Marine Oligochaeta (Video)

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75RR
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Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Marine Oligochaeta (Video)

#1 Post by 75RR » Wed Jul 18, 2018 7:14 pm

Found this worm trapped in an alga - though I would take advantage of that and try out several illumination techniques.
During the time it took to video it the worm turned around and then turned around again.
I added a couple of drops of water at the end and released it. Was hoping to capture its escape but did not manage it.

List is as follows - 20 sec of each

Plan 16x/0.35 - COL
Plan 16x/0.35 - DF
Neofluar Ph 16x/40 - Phase
Plan 16x/0.35 - Pol
Plan 16x/0.35 - DIC

Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

GaryB
Posts: 77
Joined: Tue Sep 05, 2017 10:33 am

Re: Marine Oligochaeta (Video)

#2 Post by GaryB » Thu Jul 19, 2018 12:07 am

very nice!
I like the Pol and phase best.

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ImperatorRex
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Re: Marine Oligochaeta (Video)

#3 Post by ImperatorRex » Fri Jul 20, 2018 5:35 pm

wow. Impressive comparison. I also like the darkfield!

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vasselle
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Location: France

Re: Marine Oligochaeta (Video)

#4 Post by vasselle » Fri Jul 20, 2018 6:01 pm

Bonjour
Très belle vidéo avec différentes types éclairages.
Merci pour le partage
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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coominya
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Re: Marine Oligochaeta (Video)

#5 Post by coominya » Sun Jul 22, 2018 10:41 pm

Well done 77, a nicely composed portrayal of a specimen's activity under different contrasts.
I thought the DF showed the best detail.

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75RR
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Re: Marine Oligochaeta (Video)

#6 Post by 75RR » Mon Jul 23, 2018 1:35 pm

Many thanks GaryB, ImperatorRex, vasselle and coominya
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Hobbyst46
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Re: Marine Oligochaeta (Video)

#7 Post by Hobbyst46 » Mon Jul 23, 2018 1:59 pm

Very nice photography. Well arranged. I liked the DF best.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

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coominya
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Re: Marine Oligochaeta (Video)

#8 Post by coominya » Wed Jul 25, 2018 1:05 am

Hobbyst46 wrote:Very nice photography. Well arranged. I liked the DF best.
I bought some of those cheap black half-moons and dicks to drop into my condenser filter holder but after an hour of experimentation walked away in disgust as I couldn't get a decent DF image. I'll get them out again and try some more with different settings.

Hobbyst46
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Re: Marine Oligochaeta (Video)

#9 Post by Hobbyst46 » Wed Jul 25, 2018 12:12 pm

coominya wrote:
Hobbyst46 wrote:Very nice photography. Well arranged. I liked the DF best.
I bought some of those cheap black half-moons and dicks to drop into my condenser filter holder but after an hour of experimentation walked away in disgust as I couldn't get a decent DF image. I'll get them out again and try some more with different settings.
Neither have I. I cut all circles (DIY Rheinberg) and experimented, placing them in the swing-out filter tray beneath the condenser. All failed. then forum member MicroBob explained that not all condensers and filter holders work alike in this respect, so the trick is not fool-proof.
Then, I tried placing an opaque black paper circle in one of the empty ports of the Zeiss phase contrast condenser. The circle is then as close to the iris aperture horizontal plane as can be. In fact, it is within that plane!. Now I can get some darkfield with it, with the 10x and 16x objectives. It is more or less the same as DF I get with the phase ring, PH3, as recommended by Zeiss. The minus point was that I cannot center the (hand-cut) circle within the condenser port.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

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