Fossil Diatom

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KurtM
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Fossil Diatom

#1 Post by KurtM » Thu Aug 02, 2018 9:36 pm

Been a while since I posted, but that doesn't mean I haven't been spending time in the lab. Here's a group of pix I took that tries to get all possible detail out of a diatom in order to understand the morphology.

First is through the 16x/.35 lens + 1.6x on the Optovar, DIC. The rainbow colors are naturally occurring diffraction effects, and really pretty:
7-31-18 DMap 16x_pn_lzn.jpg
7-31-18 DMap 16x_pn_lzn.jpg (155.62 KiB) Viewed 1423 times


Then, through the 40x/.65 with DIC, natural color:
7-31-18 DMap 40x_pn_lzn.jpg
7-31-18 DMap 40x_pn_lzn.jpg (144.53 KiB) Viewed 1423 times


Then converted to gray scale:
7-31-18 DMap 40x retouched_lzn_grayscale.jpg
7-31-18 DMap 40x retouched_lzn_grayscale.jpg (191.76 KiB) Viewed 1423 times


Next, colors inverted, then converted to gray scale:
7-31-18 DMap 40x retouched_pn_lzn_grayscale.jpg
7-31-18 DMap 40x retouched_pn_lzn_grayscale.jpg (170.55 KiB) Viewed 1423 times
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

GaryB
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Re: Fossil Diatom

#2 Post by GaryB » Thu Aug 02, 2018 9:41 pm

Those are very pretty. I do like the colors of the 16x and the inverted 40x

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KurtM
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Re: Fossil Diatom

#3 Post by KurtM » Thu Aug 02, 2018 9:42 pm

Next is the same through the 63x/1/4 Planapo, DIC, natural light:
7-31-18 DMap retouched 63x.jpg
7-31-18 DMap retouched 63x.jpg (208.83 KiB) Viewed 1420 times


Then with colors inverted:
7-31-18_DMap retouched_63x_pn_lzn.jpg
7-31-18_DMap retouched_63x_pn_lzn.jpg (224.87 KiB) Viewed 1420 times


And finally, colors inverted and the result converted to gray scale:
7-31-18 DMap retouched 63x pn_lzn_grayscale.jpg
7-31-18 DMap retouched 63x pn_lzn_grayscale.jpg (177.62 KiB) Viewed 1420 times


None of this was about creating the prettiest image, but to use all of them to get all possible information on this diatom's morphology. It was a fun exercise, and answered my questions. Thought y'all might enjoy the pix.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/

Hobbyst46
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Re: Fossil Diatom

#4 Post by Hobbyst46 » Thu Aug 02, 2018 10:12 pm

What I mostly like is that under the 16X, the diatom top appears flat (except for the rim), whereas under the 63X, the curvature at the top center shows - a "button" like center, and the rim is also not at the same plane. A nice demonstration of selective focusing to show the cross-sectional curvature of the top surface (lid of frustule). Thanks for posting.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

billbillt
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Re: Fossil Diatom

#5 Post by billbillt » Fri Aug 03, 2018 6:28 am

Great work.. Thanks for posting....

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75RR
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Re: Fossil Diatom

#6 Post by 75RR » Fri Aug 03, 2018 6:45 am

You have been practicing! :) Very nice work
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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vasselle
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Re: Fossil Diatom

#7 Post by vasselle » Fri Aug 03, 2018 3:29 pm

Bonjour
Superbes images
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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zzffnn
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Re: Fossil Diatom

#8 Post by zzffnn » Fri Aug 03, 2018 7:05 pm

Very nice, thank you for sharing, Kurt!

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