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 Post subject: Paramecium
PostPosted: Mon Aug 13, 2018 1:40 pm 
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Joined: Mon Aug 13, 2018 9:25 am
Posts: 4
Location: Dublin
Hi all,

As my first post into the imaging section of this forum, I would like to share with you some of my recent shots of paramecium.

Images were captured with Canon 750D on an Olympus BX51 in DIC with a x20 objective.

Apologies for the low quality but the forum will not allow me to upload a file size exceeding 500kb.


Attachments:
File comment: Paramecium formed small clusters in the vicinity of algal strands. This is one such cluster.
IMG_0049_1.jpg
IMG_0049_1.jpg [ 474.93 KiB | Viewed 925 times ]
File comment: Paramecium feeding on the biofilm that encapsulates a strand of alga. The cell nuclei and cytoskeletal network is visible in the algal cells that form the filament. It is mind blowing to watch the motor proteins streaming along the dynamic filaments that make up the network.
IMG_0070.jpg
IMG_0070.jpg [ 431.93 KiB | Viewed 925 times ]
File comment: A rootlet of an aquatic plant provides the framework for a dense ecosystem of microscopic life. We can see it covered with all kinds of algal, cyanobacteria and diatomic species. A Paramecium bursaria feeds on bacteria living in this dense forest.
IMG_0236.jpg
IMG_0236.jpg [ 382.6 KiB | Viewed 926 times ]
File comment: Here we can see Paramecium bursaria photographed as it skimmed up and down along an algal strand feeding on bacteria. It's mouth part and cilia are clearly visible aswell as the mutualistic endosymbiotic Zoochlorella (green alga), birefringent crystals and various cellular compartments. The cellulose cell walls of the alga strand exhibiting optical activity.
DPP_0018.jpg
DPP_0018.jpg [ 490.16 KiB | Viewed 926 times ]

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Last edited by Nanonaut on Mon Aug 13, 2018 8:47 pm, edited 2 times in total.
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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 3:06 pm 
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Joined: Mon Nov 10, 2014 10:01 pm
Posts: 2872
Thanks for sharing.. Those are very good..

BillT


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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 3:24 pm 
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Joined: Mon Aug 21, 2017 9:02 pm
Posts: 1969
Great photos. If possible, please share the technical details - which optics, illumination etc. Thanks!

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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 4:29 pm 
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Joined: Fri Mar 30, 2018 4:12 pm
Posts: 191
Location: Germany, Karlsruhe
Really great shots. Somehow reminds me to the old Zeiss DIC system - respectivley the color contrast image.

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sincerely Jochen
My microscopes: Zeiss inverse IM35, Zeiss Standard (RA, WL, Universal, Junior), Stemi III
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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 6:39 pm 
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Joined: Mon Aug 13, 2018 9:25 am
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Location: Dublin
Hobbyst46 wrote:
Great photos. If possible, please share the technical details - which optics, illumination etc. Thanks!


Thanks, I have added in those details now.

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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 7:09 pm 
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Joined: Sun Oct 12, 2014 2:34 am
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Location: Estepona
Very nice. Especially like the second one.
Quote:
Images were captured with Canon 750D on an Olympus BX51 in DIC at ~ x200 using flash.
I take it that means you used a 20x objective?
Could you tell/show us how the 'flash' is incorporated?

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 Post subject: Re: Paramecium
PostPosted: Mon Aug 13, 2018 10:36 pm 
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Joined: Sat Jun 18, 2016 11:55 am
Posts: 950
Location: Arnold, Missouri USA
Very nice!

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Olympus IX70 HMC
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 Post subject: Re: Paramecium
PostPosted: Tue Aug 14, 2018 12:43 am 
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Joined: Sun May 07, 2017 12:01 am
Posts: 214
Location: sunny UK
Stunning captures. :) Huge wow factor on that 2nd image.


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 Post subject: Re: Paramecium
PostPosted: Tue Aug 14, 2018 5:24 am 
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Joined: Mon Jul 16, 2018 4:48 am
Posts: 16
awesome pics dude


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 Post subject: Re: Paramecium
PostPosted: Tue Aug 14, 2018 5:10 pm 
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Joined: Mon Oct 20, 2014 7:54 pm
Posts: 1374
Beautiful subjects, fantastic image captures, a sense of how these protozoa swarm and prod substrate, and tumble from you images...thank you for this wonderful posting. Charlie Guevara, I'm fond of protozoa.


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 Post subject: Re: Paramecium
PostPosted: Thu Aug 16, 2018 12:17 am 
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Joined: Sun Mar 11, 2018 11:33 am
Posts: 279
Location: Brisbane Aust
"Bugger me (aussie expression) Those are exquisite photos nanonaut. Your composition is beautiful.


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 Post subject: Re: Paramecium
PostPosted: Sun Aug 19, 2018 4:20 pm 
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Joined: Sat Oct 25, 2014 5:32 pm
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Location: France
Bonjour
Very nice images

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