MicrobeHunter.com Microscopy Forum

Planapo 63x/1.4, DIC, 130µm, 15 image stack in Photoshop

Classic and superb.

Remarkable image! It even shows some pore detail.
You know how to use this fine objective!

Bob

I have to agree with the others... One of the best I have ever seen posted...

BillT

Beautiful 75

Very pretty specimen and image!

Yep, and I'm here to say there's nothing easy about using that lens on a living diatom! Oil top & bottom, I suppose? How on earth did you get a healthy Pleurosigma (which is what it looks like to me though there's plenty of room for error) to hold still??

Many thanks Hobbyst46, MicroBob, billbillt, rnabholz, SunshineLW and KurtM ... very kind.

Oil top & bottom, I suppose? How on earth did you get a healthy Pleurosigma (which is what it looks like to me though there's plenty of room for error) to hold still??

Yes, oil top and bottom. I reuse slides and slips so one more oily surface is hardly a problem.
He may have been partially pinned as I had extracted water (with absorbent paper) earlier trying to pin down a very wiggly nematode.
The smaller diatoms were quite active.

Superb image 75' - you my friend have become a master! As mentioned by others, I've also never seen better.
It really seems to me that you've extracted every tiny piece of performance from your setup - an extremely hard thing to achieve as we all know! A great demonstration of the art I'd say.

This how I personally like to see Diatoms, with their internals as it were, complete; so much structure and information to decode....

Keep 'em coming my friend, a real treat for us all!

John B.

Many thanks mrsonchus ... very kind, glad you like it.

Bonjour
Très belle image

Beautiful!

And I thought yesterdays 4 were stunners

Thanks for this treat of live marine diatom, 75RR. An oiled 'front and back' slide with your crisp optics is a delight for me to share in...thanks for these marine live forms.

Are you observing fresh marine samples..or a few days old samples? How do you collect your samples...from what 'habitat'..open waters, surf flats, collected matts of washed up vegetation...sand bottom...I'm curious, 75RR. Charlie guevara

Many thanks vasselle, hkv, Grahame and charlie g

Are you observing fresh marine samples..or a few days old samples? How do you collect your samples...from what 'habitat'..open waters, surf flats, collected matts of washed up vegetation...sand bottom...I'm curious, 75RR.

Collected these samples in the local Fisherman's Port/Marina. I pulled up one of the many ropes that trail in the water from the pier and plucked some alga off it. They were one day old at the time I took the photographs.
Location is in Spain, about 30 km east of Gibraltar in the Mediterranean Sea.

that is excellent work - think I might prefer the live diatom shots with the colour present, but they are all interesting subjects to photograph with some amazing images posted here.

Amazing view, seeing the dots poking through is very cool.