Genus Simocephalus

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Grahame
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Genus Simocephalus

#1 Post by Grahame » Sat Sep 08, 2018 11:15 pm

Quite a common critter in the wetland that I'm getting my water samples from.
4x acro, over 100 images in the stack, probably overkill, but it's the best internal detail I've got to date.
Genus Simocephalus.jpg
Genus Simocephalus.jpg (75.49 KiB) Viewed 1718 times

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Aenima
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Re: Genus Simocephalus

#2 Post by Aenima » Sat Sep 08, 2018 11:18 pm

thats awesome - i always struggle when focus stacking transparent subjects - all the details usually blend into a mess in the final image.

Great stuff. :)

Grahame
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Re: Genus Simocephalus

#3 Post by Grahame » Sat Sep 08, 2018 11:28 pm

Thanks Aenima
I know what you mean, I had to retouch the intestine in this image because of that.

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rnabholz
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Re: Genus Simocephalus

#4 Post by rnabholz » Mon Sep 10, 2018 5:49 pm

Very good image of a tremendously difficult subject to shoot, especially a 100 shot stack.

Did you pin him with the coverslip?

Well done.

Rod

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Re: Genus Simocephalus

#5 Post by Grahame » Sat Sep 15, 2018 7:43 am

Hi Rod,
Sorry to take so long to get back to you.
Out of the loop for a few days.
I transferred it to Glycerol.
Slows things down a bit.
I don't use it much, but find it really useful for these critters.

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Re: Genus Simocephalus

#6 Post by rnabholz » Sat Sep 15, 2018 4:20 pm

No worries, I understand.

I have never used glycerol, but have heard of its usefulness with subjects like these. I have used the pinning technique to so good effect, but it can cause some undesirable side effects....especially from the subject's point of view.

Thanks

Rod

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Re: Genus Simocephalus

#7 Post by Grahame » Sat Sep 15, 2018 11:14 pm

I make mini aquariums out of .2mm thick acetate and put the critter and the glycerol in that, then a cover slip.
The internals are still moving around but with some luck you can time the shots to have the bits in the same place each shot at the sharpness level you are working at.
I use the aquariums with the pond water as well, things seem to be able to swim freely in them.
The main prob I find with glycerol is it has a bad tendency to bubble, once you get one in the mix it's pretty much impossible to remove.

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Re: Genus Simocephalus

#8 Post by Hobbyst46 » Sun Sep 16, 2018 9:08 pm

Grahame wrote:I make mini aquariums out of .2mm thick acetate and put the critter and the glycerol in that, then a cover slip.
The internals are still moving around but with some luck you can time the shots to have the bits in the same place each shot at the sharpness level you are working at.
I use the aquariums with the pond water as well, things seem to be able to swim freely in them.
The main prob I find with glycerol is it has a bad tendency to bubble, once you get one in the mix it's pretty much impossible to remove.
Other viscous media, like fructose solution and nail polish, also release air bubbles. It is dissolved air in the liquid. The only way I know to remove bubbles is to apply vacuum to the slide. Member mrsonchus (I believe) has posted a very nice DIY vacuum chamber, which so far I failed to copy...
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Grahame
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Re: Genus Simocephalus

#9 Post by Grahame » Sun Sep 16, 2018 11:58 pm

Thanks Hobbyst46,
Went looking for the vacuum chamber and got lost in a thread on mounting dandelion stems by mrsonchus.
This site is worse than aimlessly following leads on G for getting sidetracked :)

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Re: Genus Simocephalus

#10 Post by Grahame » Mon Sep 17, 2018 7:26 am


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vasselle
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Re: Genus Simocephalus

#11 Post by vasselle » Wed Sep 19, 2018 7:38 pm

very nice
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Grahame
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Re: Genus Simocephalus

#12 Post by Grahame » Wed Sep 19, 2018 8:41 pm

Merci beaucoup vasselle

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75RR
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Re: Genus Simocephalus

#13 Post by 75RR » Sun Sep 23, 2018 5:33 am

Agree. Very nice image of a difficult subject.
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Re: Genus Simocephalus

#14 Post by Grahame » Sun Sep 23, 2018 8:54 am

Thanks 75RR

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