French Phase Contrast Diatoms

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rnabholz
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French Phase Contrast Diatoms

#1 Post by rnabholz » Tue Sep 18, 2018 1:29 am

Hadn't fired up the Phase Contrast machine for a while, so threw the switch tonight.

AO 20, 100x Dark Phase, Phase Condenser, Canon 70D, Single frames.
49/24µm
49/24µm
St Laurent La Vernede 0004 Final.jpg (135.57 KiB) Viewed 1672 times
71µm for half
71µm for half
St Laurent La Vernede 9964 Final.jpg (111.97 KiB) Viewed 1672 times
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53µm
St Laurent La Vernede 9967 Final.jpg (146.32 KiB) Viewed 1672 times
42µm
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St Laurent La Vernede 9988 Final.jpg (110.29 KiB) Viewed 1672 times
102µm for half
102µm for half
St Laurent La Vernede 9996 Final.jpg (104.43 KiB) Viewed 1672 times

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rnabholz
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Re: French Phase Contrast Diatoms

#2 Post by rnabholz » Tue Sep 18, 2018 1:32 am

More
95µm
95µm
St Laurent La Vernede 9958 Final.jpg (147.97 KiB) Viewed 1670 times
44µm
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St Laurent La Vernede 9970 Final.jpg (119.11 KiB) Viewed 1670 times
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55µm
St Laurent La Vernede 9976 Final.jpg (117.69 KiB) Viewed 1670 times
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St Laurent La Vernede 9986 Final.jpg (114.02 KiB) Viewed 1670 times

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75RR
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Re: French Phase Contrast Diatoms

#3 Post by 75RR » Tue Sep 18, 2018 1:39 am

Had forgotten about phase imaged diatoms - it makes for very attractive images.
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rnabholz
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Re: French Phase Contrast Diatoms

#4 Post by rnabholz » Tue Sep 18, 2018 1:17 pm

Thanks 75.

Phase can help dig out some details at times, and is fun for a change of pace.

Not in anyway stackable images though, so you have to make a "call" about the focus point, which opens the door to multiple options for each form. Kind of a nice exercise on occasion.

Thanks for responding

Rod

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Radazz
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Re: French Phase Contrast Diatoms

#5 Post by Radazz » Tue Sep 18, 2018 1:45 pm

Very nice phase. Pretty.
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Hobbyst46
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Re: French Phase Contrast Diatoms

#6 Post by Hobbyst46 » Tue Sep 18, 2018 2:15 pm

Great photos.
I tried stacking phase images, and the results were OK, but aesthetically not as impressive as DIC image stacks.
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KurtM
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Re: French Phase Contrast Diatoms

#7 Post by KurtM » Tue Sep 18, 2018 2:49 pm

Very nice indeed. I really enjoyed the longer string of images, and especially like the first two in the second set.
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rnabholz
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Re: French Phase Contrast Diatoms

#8 Post by rnabholz » Tue Sep 18, 2018 8:14 pm

Radazz wrote:Very nice phase. Pretty.
Thanks Radazz, count on phase for some extra pizazz.

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Re: French Phase Contrast Diatoms

#9 Post by rnabholz » Tue Sep 18, 2018 8:17 pm

Hobbyst46 wrote:Great photos.
I tried stacking phase images, and the results were OK, but aesthetically not as impressive as DIC image stacks.
Thanks Hobby.

Not much can compete with DIC! ;^)

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rnabholz
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Re: French Phase Contrast Diatoms

#10 Post by rnabholz » Tue Sep 18, 2018 8:21 pm

KurtM wrote:Very nice indeed. I really enjoyed the longer string of images, and especially like the first two in the second set.
Thanks Kurt.

That centric you liked caught my eye too. Seems to be very delicate, and shows some damage. It is the only one like it I found in 6 slides.

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KurtM
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Re: French Phase Contrast Diatoms

#11 Post by KurtM » Wed Sep 19, 2018 12:40 am

As you're probably aware, I love broken diatoms. In recent samples it's like getting a bit of a dissection thrown in that give clues as to how the thing is constructed. Fossil sample is totally different, makes you wonder what all they went through in the past million years or two. That centric makes me wonder how it got broken just that way ... those are itty bitty little holes, how did they get there? Without the rest of the shell fracturing?
Cheers,
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billbillt
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Re: French Phase Contrast Diatoms

#12 Post by billbillt » Wed Sep 19, 2018 1:21 pm

rnabholz wrote:
Hobbyst46 wrote:Great photos.
I tried stacking phase images, and the results were OK, but aesthetically not as impressive as DIC image stacks.
Thanks Hobby.

Not much can compete with DIC! ;^)
Yup.. DIC is king.....
BillT

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Re: French Phase Contrast Diatoms

#13 Post by rnabholz » Wed Sep 19, 2018 2:08 pm

KurtM wrote:As you're probably aware, I love broken diatoms. In recent samples it's like getting a bit of a dissection thrown in that give clues as to how the thing is constructed. Fossil sample is totally different, makes you wonder what all they went through in the past million years or two. That centric makes me wonder how it got broken just that way ... those are itty bitty little holes, how did they get there? Without the rest of the shell fracturing?
I thought of you while I was shooting it.

It really is amazing that these things survive at all. Tiny, intricate glass structures, encased in sedimentary rock, mined, freed by acid, mounted at 350 degrees F. How the aren't ALL broken is a bit of a miracle.

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Re: French Phase Contrast Diatoms

#14 Post by billbillt » Wed Sep 19, 2018 5:54 pm

KurtM wrote:As you're probably aware, I love broken diatoms. In recent samples it's like getting a bit of a dissection thrown in that give clues as to how the thing is constructed. Fossil sample is totally different, makes you wonder what all they went through in the past million years or two. That centric makes me wonder how it got broken just that way ... those are itty bitty little holes, how did they get there? Without the rest of the shell fracturing?
Hi Kurt,

This may be the answer to the "holes" in diatoms...

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221735/

BillT

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Re: French Phase Contrast Diatoms

#15 Post by rnabholz » Wed Sep 19, 2018 6:23 pm

Here is a discussion we had a while back about a similar situation :

viewtopic.php?f=6&t=3349

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vasselle
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Re: French Phase Contrast Diatoms

#16 Post by vasselle » Wed Sep 19, 2018 7:35 pm

Bonjour
Très belles réalisation avec de très belles iamges
Cordialement seb
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