copepod nauplius

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75RR
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copepod nauplius

#1 Post by 75RR » Sat Sep 22, 2018 7:26 am

Plan 40x/0.65, DIC, 180µm, 3 stack stitch in Photoshop
Pinned by cover slip. Cleaned up background
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Grahame
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Re: copepod nauplius

#2 Post by Grahame » Sat Sep 22, 2018 10:33 am

Yet another amazing image 75RR :D

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KurtM
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Re: copepod nauplius

#3 Post by KurtM » Sun Sep 23, 2018 12:48 am

Yep, that's a breathtaking DIC effect alright! Amazing!! :shock: 8-)

It's doubly wonderful for me since I have the same optics on my bench, what better inspiration than that?
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vasselle
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Re: copepod nauplius

#4 Post by vasselle » Sun Sep 23, 2018 2:56 am

Bonjour
Superbe image en DIC.
Bravo pour votre travail que j'apprécie.
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

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75RR
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Re: copepod nauplius

#5 Post by 75RR » Sun Sep 23, 2018 5:19 am

Many thanks Grahame, KurtM and vasselle. Very kind.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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coominya
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Re: copepod nauplius

#6 Post by coominya » Fri Sep 28, 2018 11:00 pm

75RR wrote:Plan 40x/0.65, DIC, 180µm,
Pinned by cover slip.
When you say 'pinned' 75, do you imply you squeezed the slip down to sort of immobilize the copepod? I washed some slides last week and there must have been a residue of detergent left on them because the water sample spread very thin even before I placed the slip on. It didn't seem to kill my subjects but they certainly would have had less room to move around in.

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75RR
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Re: copepod nauplius

#7 Post by 75RR » Sat Sep 29, 2018 4:45 am

When you say 'pinned' 75, do you imply you squeezed the slip down to sort of immobilize the copepod?
I use it to mean that the subject, in this case the copepod, is held down by the weight of the cover slip.
This will occur naturally via evaporation, provided there is not some detritus to hold the cover slip up.
One can accelerate the process using absorbent paper held to the edges of the cover slip or as you say pressing down on it.
However, pressing the cover slip down is a delicate business, as it is hard to gauge how much pressure to apply.
On the other hand two dimensional subjects are easier to photograph. ;)

A pinned subject can be released by adding a drop of water to the edge of the cover slip.
This is useful if the position it was pinned in was not ideal and we want to make another attempt or when we want to extend the viewing time of the wet slide.

Additionally, the higher the magnification of the objective the shallower the depth of field and the smaller the working distance, so with high power objectives, the less water between the subject and the cover slip the better. See tables:
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Depth of field.png
Depth of field.png (38.39 KiB) Viewed 1186 times
Last edited by 75RR on Sat Sep 29, 2018 5:45 am, edited 2 times in total.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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75RR
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Re: copepod nauplius

#8 Post by 75RR » Sat Sep 29, 2018 4:47 am

Sorry - double post
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
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coominya
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Re: copepod nauplius

#9 Post by coominya » Sat Sep 29, 2018 11:10 am

That's quite a jump in DOF between 40x and 20x, the two objectives I use the most, and explains why my view at the higher power is diminished in terms of focus. Thanks for the tips on adding and removing water.

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