Photo stacking transparent objects

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waterman
Posts: 28
Joined: Sun Oct 14, 2018 12:26 pm
Location: UK

Photo stacking transparent objects

#1 Post by waterman » Sun Oct 21, 2018 7:01 am

All

When scanning a slide at 400x, I caught glimpses of a ghostly outline of what I thought was a daphnia case, however, after taking about 20 photographs and using a stacking program it revealed the following.
On transparent objects photo stacking really doesn't work very well and while I'm not sure of the identification anymore, I love the Picasso type effect.

Imagedead by [url=https://www.flickr.com/photos/144618088@N07/]

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75RR
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Re: Photo stacking transparent objects

#2 Post by 75RR » Sun Oct 21, 2018 7:19 am

When stacking transparent objects one has to be careful not to focus too deeply.
Look through the images you took and stack only those that show the subject you wish to capture.
Note: If the alga is above your subject then getting an image without it will be difficult.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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SunshineLW
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Re: Photo stacking transparent objects

#3 Post by SunshineLW » Wed Nov 21, 2018 10:12 am

I've stacked objects larger than 100-200 micrometers thick. It is possible, but it can take hours to re-touch the final image, selecting only the areas of each image in the image stack that you wish to appear in the final stacked image. It is an unusual art consisting of both photography and digital painting.

Here are several of my most prized stacked images of transparent specimens:
- Notoedres sp. mite: https://www.veterinaryparasitology.com/notoedres.html
- Lynxacarus radovskyi mite: https://www.veterinaryparasitology.com/ ... vskyi.html
- Ornithonyssus sylviarum mite: https://www.veterinaryparasitology.com/ ... yssus.html
- Entamoeba histolytica trophozoite: https://www.veterinaryparasitology.com/entamoeba.html

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