Photo stacking transparent objects

[waterman]

All

When scanning a slide at 400x, I caught glimpses of a ghostly outline of what I thought was a daphnia case, however, after taking about 20 photographs and using a stacking program it revealed the following.
On transparent objects photo stacking really doesn't work very well and while I'm not sure of the identification anymore, I love the Picasso type effect.

dead by [url=https://www.flickr.com/photos/144618088@N07/]

[75RR]

When stacking transparent objects one has to be careful not to focus too deeply.
Look through the images you took and stack only those that show the subject you wish to capture.
Note: If the alga is above your subject then getting an image without it will be difficult.

[SunshineLW]

I've stacked objects larger than 100-200 micrometers thick. It is possible, but it can take hours to re-touch the final image, selecting only the areas of each image in the image stack that you wish to appear in the final stacked image. It is an unusual art consisting of both photography and digital painting.

Here are several of my most prized stacked images of transparent specimens:
- Notoedres sp. mite: https://www.veterinaryparasitology.com/notoedres.html
- Lynxacarus radovskyi mite: https://www.veterinaryparasitology.com/ ... vskyi.html
- Ornithonyssus sylviarum mite: https://www.veterinaryparasitology.com/ ... yssus.html
- Entamoeba histolytica trophozoite: https://www.veterinaryparasitology.com/entamoeba.html