Cyanobacteria - Gloeotrichia

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hkv
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Cyanobacteria - Gloeotrichia

#1 Post by hkv » Thu Oct 25, 2018 8:04 pm

Long time since I posted in this forum so I thought I would share a few images. I was out boating in august and noticed some small "algae" floating on the surface of the water. It was like small hairy balls and I took a sample in a beer can to check them out. To my surprise, they were not algae at all, but cyanobacterias. In fact, Gloeotrichia in colonial form. A very nice looking species, but hard to image due to all the hairs. Stacking is a pain due to artifacts in the final image which takes some manual work to remove. Some of these are stacked in several steps with the center in one go and the outer parts in a separate go. In the fluorescent images I have stained them with Acridine Orange for a few minutes. The inner part got less stain and remained green, whereas the outer hairs sucked up more of the staining fluid. Then rinsed in fresh water before imaging.

Click on the images to get more information about the technique and objectives used.


Darkfield:

Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr

DIC:

Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr

Brightfield:

Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr

Fluo:
Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr

Fluo:

Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr

Fluo:

Image
Cyanobacteria - Gloeotrichia by Håkan Kvarnström, on Flickr
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MichaelG.
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Re: Cyanobacteria - Gloeotrichia

#2 Post by MichaelG. » Thu Oct 25, 2018 8:35 pm

Spectacular images

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75RR
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Re: Cyanobacteria - Gloeotrichia

#3 Post by 75RR » Thu Oct 25, 2018 9:08 pm

Agree! An excellent series.
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Re: Cyanobacteria - Gloeotrichia

#4 Post by Hobbyst46 » Thu Oct 25, 2018 9:21 pm

Great photos. To my taste the DF is nicest...
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KurtM
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Re: Cyanobacteria - Gloeotrichia

#5 Post by KurtM » Fri Oct 26, 2018 12:24 am

A pain to image, maybe, but the results are spectacular! Don't be a stranger, your images are always so very nice.
Cheers,
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Re: Cyanobacteria - Gloeotrichia

#6 Post by rnabholz » Fri Oct 26, 2018 1:57 am

Your commitment to go the extra mile shows through in the spectacular results. Just beautiful, well done

Rod

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hkv
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Re: Cyanobacteria - Gloeotrichia

#7 Post by hkv » Fri Oct 26, 2018 7:20 am

Thank you all for your comments! The darkfield image is also one of my favourite.
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Radazz
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Re: Cyanobacteria - Gloeotrichia

#8 Post by Radazz » Fri Oct 26, 2018 12:55 pm

Very beautiful images!
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zzffnn
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Re: Cyanobacteria - Gloeotrichia

#9 Post by zzffnn » Fri Oct 26, 2018 1:03 pm

Great images!
Advantage of fluorescence imaging can be clearly seen with this subject.

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Re: Cyanobacteria - Gloeotrichia

#10 Post by Grahame » Tue Oct 30, 2018 6:48 am

Neat images.
Have you tried a cross-eye stereo of any of them ?

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hkv
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Re: Cyanobacteria - Gloeotrichia

#11 Post by hkv » Tue Oct 30, 2018 7:10 am

Grahame wrote:Neat images.
Have you tried a cross-eye stereo of any of them ?
No, I have not tried that. I have to struggle to see cross-eye stereo at all and mostly fail in doing so... Therefore, I never enable that feature when stacking. Would be neat to see these in 3D for sure.
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Re: Cyanobacteria - Gloeotrichia

#12 Post by MicroBob » Tue Oct 30, 2018 7:40 am

Hi Håkan,

really great images! Every time I see how beautiful and detailed nature is it becomes more and more difficult to understand how badly we treat it.

It's nice of you to decribe the amount of work and capability that was neede to make these images. So we are not disappointed when we can't get anywhere near! :lol:

Bob

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hkv
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Re: Cyanobacteria - Gloeotrichia

#13 Post by hkv » Tue Oct 30, 2018 9:04 am

MicroBob wrote:Hi Håkan,

really great images! Every time I see how beautiful and detailed nature is it becomes more and more difficult to understand how badly we treat it.

It's nice of you to decribe the amount of work and capability that was neede to make these images. So we are not disappointed when we can't get anywhere near! :lol:

Bob
Bob, thanks! I agree. It i amazing to see how beautiful nature is. Especially when looking close. Also fascinating to understand that protists together make up 2 times as much biomass as all animals in the world. If we include bacteria, the biomass is 40 times more than of animals. And these are hidden to the naked eye unless we make them visible using our instruments!
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Aenima
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Re: Cyanobacteria - Gloeotrichia

#14 Post by Aenima » Tue Oct 30, 2018 2:54 pm

wow - awesome shots - really like them

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Re: Cyanobacteria - Gloeotrichia

#15 Post by miguel5526 » Tue Oct 30, 2018 3:41 pm

muy buenas tomas FELICITACIONES!!

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Re: Cyanobacteria - Gloeotrichia

#16 Post by Tarloth » Fri Nov 02, 2018 11:46 pm

Fantastic photographs!

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Re: Cyanobacteria - Gloeotrichia

#17 Post by RobBerdan » Fri Nov 23, 2018 1:32 am

Beautiful photomicrographs well done.

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hkv
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Re: Cyanobacteria - Gloeotrichia

#18 Post by hkv » Fri Nov 23, 2018 8:37 pm

Thanks! I appreciate your feedback!
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