Biddulphia pulchella (provisional ID)

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75RR · #1 Post by **75RR** » Sat Nov 10, 2018 8:31 pm

Plan 40x/0.65, DIC, 140µm, 46 image stack in Photoshop
Plan 16x/0.35, DIC, 140µm (3rd diatom, left to right), 48 image stack in Photoshop

MicroBob · #2 Post by **MicroBob** » Sun Nov 11, 2018 5:32 pm

Great images , thank you for showing!

How does DIC combine with stacking? I think DIC is said to emphasize the structure detail of one single layer. This should be an advantage for stacking. What is your impression? Do you need more images in a stack?

Bob

75RR · #3 Post by **75RR** » Sun Nov 11, 2018 7:22 pm

Thanks MicroBob

DIC stacks well in my experience. I believe it imparts a shallower depth of field so it tends to require more images and hence larger stacks.
It can therefore be a little frustrating when the subject moves again (having finally paused for a short rest) just before you finish taking the final remaining images to complete the stack.

KurtM · #4 Post by **KurtM** » Mon Nov 12, 2018 4:44 am

Very nice images of a very nice subject! Here ya go:

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Biddulphia pulchella (provisional ID)

Biddulphia pulchella (provisional ID)

Re: Biddulphia pulchella (provisional ID)

Re: Biddulphia pulchella (provisional ID)

Re: Biddulphia pulchella (provisional ID)