Pleurosigma + 2 Anisonema vitrea

[75RR]

Planapo 63x/1.4, DIC, 210µm, 3 stacks stitched in Photoshop



Here is the image I used to ID Anisonema vitrea (As always, happy to be corrected if needed)

[Hobbyst46]

As always - such fine, impressive details ! is the diatom alive, or cleaned?

[75RR]

Thanks Hobbyst46,

not cleaned but it is from a marine sample that is a few days old (which pongs a bit) - not sure if there was anyone home at the time.

[desertrat]

Outstanding image!

If that's P. Angulatum, that's one of diatoms I like to view on Klaus Kemp's 8 form test plate.

For me, the pores (punctae?) are less easily resolvable, but that's because the highest N.A. objective I regularly use is 1.25, and the only objective I have that's higher, at 1.30, is an antique Leitz 1/12 Oel.

What was your diatom specimen mounted in?

[75RR]

Thanks desertrat,

What was your diatom specimen mounted in?

Not permanently mounted, just a temporary wet slide.

This is the sample jar:

[desertrat]

What I meant to say, was that 1.4 N.A. objective must be oil immersion to have an N.A. that high?

I never thought about using oil immersion on a temporary wet mount, but you've shown it's definitely possible.

Did you oil the condenser to the slide also?

[rnabholz]

Oh my...........

Words fail.

You are killing it 75.

[75RR]

What I meant to say, was that 1.4 N.A. objective must be oil immersion to have an N.A. that high?

I never thought about using oil immersion on a temporary wet mount, but you've shown it's definitely possible.

Did you oil the condenser to the slide also?

Sorry, my mistake. Yes oil, top and bottom! In for a penny as they say ...
Only downside to using oil on a wet slide is if the subject is near the edge ... it can seep in and lift the slide.
Not something you want with the Planapo 63x/1.4's working distance

[75RR]

too kind rnabholz ... thanks