I have been fooling around with mini-aquariums again, and for today's amusement tried my hand at imaging a larger critter than I normally tackle: a Gammarus. I'm not up on my amphipod crustaceans, and there are a zillion of these things, so I leave species to the experts. These images are stitches of 6 images each, the source images are single frames, all shot through the 4x objective on the Diaphot TMD. The result are in bright field, DIC (kinda, the 4x doesn't have a matched prism), simple POL, and POL with 1st order red compensator.
The POL images highlight musculature, of course. Overall length from stem to stern is right at 4 mm.
Gammarus spp In Different Lights
Gammarus spp In Different Lights
- Attachments
-
- 01 Gammarus spp bright field 4x.jpg (108.43 KiB) Viewed 2922 times
-
- 02 Gammarus spp DIC 4x.jpg (102.51 KiB) Viewed 2922 times
-
- 03 Gammarus spp pol 4x.jpg (53.69 KiB) Viewed 2922 times
-
- 04 Gammarus spp lambda 4x.jpg (86.23 KiB) Viewed 2922 times
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Re: Gammarus spp In Different Lights
Nice set! Like the mix of illumination techniques.
4x does have a nice depth of field!
Have you seen Cactusdave's posts on mismatch DIC objectives?
https://www.photomacrography.net/forum/ ... hlight=dic
4x does have a nice depth of field!
What prism did you substitute for the missing 4x one?The result are in bright field, DIC (kinda, the 4x doesn't have a matched prism) ...
Have you seen Cactusdave's posts on mismatch DIC objectives?
https://www.photomacrography.net/forum/ ... hlight=dic
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Gammarus spp In Different Lights
Interesting fellow, don't believe I have ever seen one before.
Nice study in illumination effects too.
Nice study in illumination effects too.
Re: Gammarus spp In Different Lights
Rod, I'll bet you have seen these guys before, probably took 'em for some sort of insect larvae zipping around. I believe they're fairly ubiquitous in FW pond/river/birdbath samples. Big enough to be easy naked-eye visible, this one is 4 mm long and as I'm sure you know that's 1/8" ... and we old draftsmen typically made the lettering on our drawings 1/8". For whatever reason, lately I've been taking increasing interest in the larger denizens of FW samples, particularly cladocera. This amphipod, plus a few of his buddies, were in a sample I was about to discard.
75, you bet I've seen Cactus Dave's article, and have studied it quite a lot as I'm sure you have too. I also once saw where Charles Krebs mentioned occasionally mismatching prisms on purpose to get improved DIC effect on the camera sensor. So I've learned to experiment, even trying prisms upside-down where possible (Zeiss but not Nikon). In the TMD's case, the prism for the 10x lens works best with the 4x; the others present an increasingly narrow, abrupt extinction band.
75, you bet I've seen Cactus Dave's article, and have studied it quite a lot as I'm sure you have too. I also once saw where Charles Krebs mentioned occasionally mismatching prisms on purpose to get improved DIC effect on the camera sensor. So I've learned to experiment, even trying prisms upside-down where possible (Zeiss but not Nikon). In the TMD's case, the prism for the 10x lens works best with the 4x; the others present an increasingly narrow, abrupt extinction band.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/