Practising microscopy techniques on sauerkraut bacteria.

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lperepol
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Joined: Mon Dec 17, 2018 3:51 am
Location: Castlegar

Practising microscopy techniques on sauerkraut bacteria.

#1 Post by lperepol » Fri Dec 28, 2018 9:05 am

Should posts like these be posted in the sandbox?

Practising microscopy techniques on bacteria. Heat fixing, oil immersion, methylene blue stain (staining).
Image one seems like I was looking at yeast cells. Over time the bacteria seem to have gotten smaller? And seems like I could focus for a better image earlier on. My skills may be deteriorating. Or something is happening with the sauerkraut.

Resolution of the images is a major dissapointment. Not sure if my techniques can be improved?

Not what I am seeing:
"The best quality sauerkraut is fermented for a minimum of 20 days. This time span ensures good flavor development, proper acidity level and complete consumption of all the sugars in the cabbage. The L. mesenteroides do most of their work in the first 3 days. They are the smallest of the three bacteria studied."
https://www.makesauerkraut.com/how-long ... auerkraut/


1) After 5 Days of Fermentation (At room temperature)
Image

2) After 17 Days of Fermentation (At room temperature)
Image


3) After 17 Days of Fermentation (At room temperature)
Image


Last edited by lperepol on Fri Dec 28, 2018 4:06 pm, edited 2 times in total.
1) OMAX 40X-2500X 18MP USB3 Plan Phase Contrast Trinocular LED with Turret Phase Disk
2) AmScope Trinocular Stereo, 3.5X-90X Magnification Four-Zone LED Ring Light

User avatar
lperepol
Posts: 100
Joined: Mon Dec 17, 2018 3:51 am
Location: Castlegar

Sauerkraut bacteria goes hi-tech

#2 Post by lperepol » Fri Dec 28, 2018 2:56 pm

https://www.ncbi.nlm.nih.gov/pmc/articl ... dMIKEb68IY
:D

Does not mention when or how Sauerkraut goes sideways though.
1) OMAX 40X-2500X 18MP USB3 Plan Phase Contrast Trinocular LED with Turret Phase Disk
2) AmScope Trinocular Stereo, 3.5X-90X Magnification Four-Zone LED Ring Light

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