Blood

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janvangastel
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Blood

#1 Post by janvangastel » Tue Jan 01, 2019 10:17 am

I made this video of a drop of blood donated by my girlfriend. I thought I would see more or less round red blood cells but what I saw is very different from that. What is visible in this video? Is my girlfriend an alien?


Hobbyst46
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Re: Blood

#2 Post by Hobbyst46 » Tue Jan 01, 2019 10:47 am

Is this a photo of fresh human blood? very different from what I have seen... perhaps you might post single images at a higher magnification.
Normal human RBC's are discs, diameter 7 micrometers appr.
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Re: Blood

#3 Post by MichaelG. » Tue Jan 01, 2019 11:53 am

Hobbyst46 wrote:... perhaps you might post single images at a higher magnification.
Normal human RBC's are discs, diameter 7 micrometers appr.
Yes, Jan, please do

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75RR
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Re: Blood

#4 Post by 75RR » Tue Jan 01, 2019 12:40 pm

Is that a petri dish? It rather looks as if you focused at the wrong depth.

It is best practice to start with a lower magnification objective (find subject - apply Köhler illumination) and then work your way up to the one you want when the objectives are parfocal, as yours are.
Is my girlfriend an alien?
She is with you ;)
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Re: Blood

#5 Post by janvangastel » Tue Jan 01, 2019 1:18 pm

Is that a petri dish? It rather looks as if you focused at the wrong depth.
No, it is a drop of blood on a glass strip with a cover slip, magnified 500x. I also tried lower maginfications and tried other focus posititions, but without any change (except for less sharpness). I will try again today.

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75RR
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Re: Blood

#6 Post by 75RR » Tue Jan 01, 2019 2:12 pm

No, it is a drop of blood on a glass strip with a cover slip, magnified 500x. I also tried lower maginfications and tried other focus posititions, but without any change (except for less sharpness). I will try again today.
I can actually see the platelets, they look to be at a lower focus level.
Start with your lowest magnification objective - move the stage up and down until you see something pink!

You also need to clean your cover slips :)
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Re: Blood

#7 Post by Crater Eddie » Tue Jan 01, 2019 3:46 pm

Wet blood samples can be less than exciting. Look up how to do a smear and stain, then you can see some interesting things.
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Re: Blood

#8 Post by janvangastel » Tue Jan 01, 2019 6:02 pm

Maybe there was not enough blood on the glass. I made a new sample, with a drop of my own blood. I think it looks better.


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Re: Blood

#9 Post by 75RR » Tue Jan 01, 2019 6:14 pm

Well done.

Hand holding a camera is quite difficult. Have you decided how/where you will attach the camera and whether you will use direct projection or afocal?
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Re: Blood

#10 Post by janvangastel » Tue Jan 01, 2019 6:40 pm

I have ordered a cheap adapter for direct projection (without camera lens). It will take some time to arrive though: somewhere between January 8th and Februari 8th The video above was made afocal. I have much experience in afocal bird photography through my Swarovski spotting scope (named digiscoping), so I am thinking about making an adapter for my microscope for afocal photography as well. The eyepieces of my Orthoplan though, do not fit into the vertical camera opening, so I will have to place the camera behind one of the visual eyepieces. Or find an eyepiece that does fit.

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Re: Blood

#11 Post by 75RR » Tue Jan 01, 2019 8:51 pm

The eyepieces of my Orthoplan though, do not fit into the vertical camera opening, so I will have to place the camera behind one of the visual eyepieces. Or find an eyepiece that does fit.
I believe the Orthplan takes 30mm eyepieces but the phototube takes the more common 23mm.
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Re: Blood

#12 Post by redflanker » Wed Jan 02, 2019 7:29 am


This is human blood under microscope that I take the video with phase contrast 100x with out oil 2 days ago.
and could you teach me how to post youtube vedio on this forum?
Last edited by redflanker on Wed Jan 02, 2019 6:24 pm, edited 3 times in total.
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Re: Blood

#13 Post by janvangastel » Wed Jan 02, 2019 10:54 am

That's a very nice video! Is this also blood between a glass strip and a cover slip?
To get a video on the forum, click the youtube button above the message window, which will give [yoube][/youtube]. Then paste only the last part of the video address between the two 'youtubes' , so for your video only the text: zUnwnt6VW5U. That will do it.

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Re: Blood

#14 Post by 75RR » Wed Jan 02, 2019 11:14 am

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Re: Blood

#15 Post by redflanker » Wed Jan 02, 2019 6:13 pm

janvangastel wrote:That's a very nice video! Is this also blood between a glass strip and a cover slip?
To get a video on the forum, click the youtube button above the message window, which will give [yoube][/youtube]. Then paste only the last part of the video address between the two 'youtubes' , so for your video only the text: zUnwnt6VW5U. That will do it.
yes,under normal 0 .17mm cover glass.
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Re: Blood

#16 Post by redflanker » Wed Jan 02, 2019 6:13 pm

thank you, Is that you in video? :D
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Re: Blood

#17 Post by 75RR » Wed Jan 02, 2019 6:31 pm

thank you, Is that you in video?
Nope, I am scared of needles!
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Re: Blood

#18 Post by redflanker » Wed Jan 02, 2019 6:49 pm

75RR wrote:
thank you, Is that you in video?
Nope, I am scared of needles!
me too,I collect the blood from acne that on my wife's face.
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Re: Blood

#19 Post by drasbell » Thu Jan 03, 2019 12:45 am

redflanker wrote:
thank you, Is that you in video? :D
I'm sure if your follow this procedure you'll have better results.
your first video looks to be focused on your cover slip. the second one looks OK, but the blood is to thick. If you try a drop of blood with a drop of water and mix with a needle, might work to thin things out . I don't now I've always done smears then stain.

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Re: Blood

#20 Post by janvangastel » Thu Jan 03, 2019 6:48 am

Thanks, I will try again shortly.

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