MicrobeHunter.com Microscopy Forum

A second Stentor video, this time in dark ground (and with the music credited). Stentors from culture.

Nice work !!

MichaelG.

Very nice video!
Thanks for sharing,
Radazz

Very nice!

Objective?

Great video!... Thanks for sharing...

BillT

2.5 x objective, plan, na 0.8

SpocksSister wrote:2.5 x objective, plan, na 0.8

I am guessing Plan 2.5 x NA 0.08 ? Do you use a condenser with this objective? how is dark field created ? it is trans-illumination, not epi-, right ?

WOW! Very nice

Bravo, spoke-sister, for this crips videow, thanks. I love my Nikon Plan 2X 160 tl objective. I can tease a darkfield contrast out of this objective with either my Nikons DF condenser setting, or by using this Plan 2X objective with one of the dl-phase condenser settings ( I forget which phase stop as I sit here and tap keys to your wonderful sharing of a huge ciliate, spock sister).

What water film sweet-spot /water film thickness under your cover-slip do you predictably maintain? I ask as there seems no drift above, or below the plane of crisp focus for the smaller active ciliates in your posted video. Then too, the depth of focus is greater with a low (2.5X objective) magnification objective...hmmm.

These noble organisms, stentors, under Vance Tartars surgical manipulations offered much understanding about body maintenance in single cell organisms. Your stentors, spock sister seem large enough to 'pat on their flanks' for good behaviors in your videow.

You term the contrast method: 'dark ground'...at 2X-10X objective magnifications...I often sense with live organisms...dark field contrast better than : '1 dl phase contrast setting'...hmmm.


Thanks for these shared encounters. Charlie guevara

Excellent music- it captured the soul of the stentor.