Aeolosoma sp.

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75RR
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Aeolosoma sp.

#1 Post by 75RR » Thu Feb 07, 2019 9:30 pm

Freshwater sample from a small brook - nice to see one again.

Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

geos
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Re: Aeolosoma sp.

#2 Post by geos » Thu Feb 07, 2019 10:31 pm

The resolution is astounding.

Tom Jones
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Re: Aeolosoma sp.

#3 Post by Tom Jones » Fri Feb 08, 2019 1:36 am

Nice. It's even budding!

einman
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Re: Aeolosoma sp.

#4 Post by einman » Fri Feb 08, 2019 1:39 am

I agree -very nice resolution.

JMK
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Re: Aeolosoma sp.

#5 Post by JMK » Fri Feb 08, 2019 2:44 am

VIBRANT !
Thanks for sharing

John

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75RR
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Re: Aeolosoma sp.

#6 Post by 75RR » Fri Feb 08, 2019 7:07 am

Many thanks geos, Tom Jones, einman and JMK

Here is a linked drawing that shows budding paratomic fission a little more clearly. https://en.wikipedia.org/wiki/Paratomy

Image
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Timor
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Re: Aeolosoma sp.

#7 Post by Timor » Fri Feb 15, 2019 9:10 am

Beautiful images!
Was there anything special about the illumination, if I may ask? Or is this just 'as it comes'.

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75RR
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Re: Aeolosoma sp.

#8 Post by 75RR » Fri Feb 15, 2019 7:41 pm

Thanks Timor,

The second part of the video, using the 16x objective, was done with DIC (Differential Interference Contrast) see link below:

The first part, using the 6.3x objective, only utilized the bottom Wollaston Prism as I lack the top one. (Not sure what to call that combination)

https://www.microscopyu.com/pdfs/Lassle ... 9-2006.pdf
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Timor
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Re: Aeolosoma sp.

#9 Post by Timor » Fri Feb 15, 2019 9:39 pm

Thanks for the information, 75RR.
Tim

Hobbyst46
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Re: Aeolosoma sp.

#10 Post by Hobbyst46 » Fri Feb 15, 2019 9:42 pm

75RR wrote:...The second part of the video, using the 16x objective, was done with DIC (Differential Interference Contrast) see link below:...
https://www.microscopyu.com/pdfs/Lassle ... 9-2006.pdf
Thanks 75RR for the interesting video clip and for the link.

Incidentally, here is a citation from the linked article about DIC microscopy:

"Objectives
Theoretically any objectives can be used, but in
practice higher grade objectives (Fluorite and
apochromatic types) are generally specified to
benefit from the high-resolution potential. In
many cases phase-contrast fluorite objectives
are chosen, permitting brightfield, DIC, phase
contrast and fluorescence observation with a
single set of objectives
.


He apparently refers to Olympus optics. Not long ago, a forum member purchased and posted a Zeiss Universal with Neofluar phase contrast optics, and the question was, would the PC objectives work for DIC.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

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75RR
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Re: Aeolosoma sp.

#11 Post by 75RR » Sat Feb 16, 2019 6:34 am

Hobbyst46 wrote:... Not long ago, a forum member purchased and posted a Zeiss Universal with Neofluar phase contrast optics, and the question was, would the PC objectives work for DIC.
Yes I remember. Poster had not received the microscope yet so he was unable to tell us about all the bits it came with.
So there is some doubt as to which of the four 160 tube DIC systems would be easiest for him to get.
I don't doubt he will be asking about DIC again once he knows exactly what he has.


Here is a link to the four Zeiss 160 DIC sistems. I have the last one.

As you can see in the 4th system the DIC sliders are designed to work with specific objectives - mostly 'Plan'

viewtopic.php?f=15&t=1543


However a bit of testing has shown that good results can sometimes be had with what some call mismatched objectives (i.e. non designated objectives)

I have not tried my luck however with Phase Contrast objectives - perhaps I should.

https://www.photomacrography.net/forum/ ... hlight=dic

These are the sliders I have. As you can see they are objective specific ...
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
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Re: Aeolosoma sp.

#12 Post by redflanker » Thu Feb 21, 2019 4:49 am

I thought the most difficult part is capture the creature and foucs on them, some of the water life move really fast, and it's hard to keep fous on them.
Microscope:Zeiss Primo star with phase contrast
Camera:Cannon 90D
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