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Aeolosoma sp.

Posted: Thu Feb 07, 2019 9:30 pm
by 75RR
Freshwater sample from a small brook - nice to see one again.


Re: Aeolosoma sp.

Posted: Thu Feb 07, 2019 10:31 pm
by geos
The resolution is astounding.

Re: Aeolosoma sp.

Posted: Fri Feb 08, 2019 1:36 am
by Tom Jones
Nice. It's even budding!

Re: Aeolosoma sp.

Posted: Fri Feb 08, 2019 1:39 am
by einman
I agree -very nice resolution.

Re: Aeolosoma sp.

Posted: Fri Feb 08, 2019 2:44 am
by JMK
VIBRANT !
Thanks for sharing

John

Re: Aeolosoma sp.

Posted: Fri Feb 08, 2019 7:07 am
by 75RR
Many thanks geos, Tom Jones, einman and JMK

Here is a linked drawing that shows budding paratomic fission a little more clearly. https://en.wikipedia.org/wiki/Paratomy

Image

Re: Aeolosoma sp.

Posted: Fri Feb 15, 2019 9:10 am
by Timor
Beautiful images!
Was there anything special about the illumination, if I may ask? Or is this just 'as it comes'.

Re: Aeolosoma sp.

Posted: Fri Feb 15, 2019 7:41 pm
by 75RR
Thanks Timor,

The second part of the video, using the 16x objective, was done with DIC (Differential Interference Contrast) see link below:

The first part, using the 6.3x objective, only utilized the bottom Wollaston Prism as I lack the top one. (Not sure what to call that combination)

https://www.microscopyu.com/pdfs/Lassle ... 9-2006.pdf

Re: Aeolosoma sp.

Posted: Fri Feb 15, 2019 9:39 pm
by Timor
Thanks for the information, 75RR.
Tim

Re: Aeolosoma sp.

Posted: Fri Feb 15, 2019 9:42 pm
by Hobbyst46
75RR wrote:...The second part of the video, using the 16x objective, was done with DIC (Differential Interference Contrast) see link below:...
https://www.microscopyu.com/pdfs/Lassle ... 9-2006.pdf
Thanks 75RR for the interesting video clip and for the link.

Incidentally, here is a citation from the linked article about DIC microscopy:

"Objectives
Theoretically any objectives can be used, but in
practice higher grade objectives (Fluorite and
apochromatic types) are generally specified to
benefit from the high-resolution potential. In
many cases phase-contrast fluorite objectives
are chosen, permitting brightfield, DIC, phase
contrast and fluorescence observation with a
single set of objectives
.


He apparently refers to Olympus optics. Not long ago, a forum member purchased and posted a Zeiss Universal with Neofluar phase contrast optics, and the question was, would the PC objectives work for DIC.

Re: Aeolosoma sp.

Posted: Sat Feb 16, 2019 6:34 am
by 75RR
Hobbyst46 wrote:... Not long ago, a forum member purchased and posted a Zeiss Universal with Neofluar phase contrast optics, and the question was, would the PC objectives work for DIC.
Yes I remember. Poster had not received the microscope yet so he was unable to tell us about all the bits it came with.
So there is some doubt as to which of the four 160 tube DIC systems would be easiest for him to get.
I don't doubt he will be asking about DIC again once he knows exactly what he has.


Here is a link to the four Zeiss 160 DIC sistems. I have the last one.

As you can see in the 4th system the DIC sliders are designed to work with specific objectives - mostly 'Plan'

viewtopic.php?f=15&t=1543


However a bit of testing has shown that good results can sometimes be had with what some call mismatched objectives (i.e. non designated objectives)

I have not tried my luck however with Phase Contrast objectives - perhaps I should.

https://www.photomacrography.net/forum/ ... hlight=dic

These are the sliders I have. As you can see they are objective specific ...

Re: Aeolosoma sp.

Posted: Thu Feb 21, 2019 4:49 am
by redflanker
I thought the most difficult part is capture the creature and foucs on them, some of the water life move really fast, and it's hard to keep fous on them.