I thinl I am getting a bit better detail on nematodes.

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
lperepol
Posts: 100
Joined: Mon Dec 17, 2018 3:51 am
Location: Castlegar

I thinl I am getting a bit better detail on nematodes.

#1 Post by lperepol » Wed Jul 31, 2019 11:15 am

The 100x objectives Phase and Standard do not seem to resolves as well as the lower magnification objectives on the Omax.

Image

Image

Image

Image

Image

Image

Image

Image

Image

Image
1) OMAX 40X-2500X 18MP USB3 Plan Phase Contrast Trinocular LED with Turret Phase Disk
2) AmScope Trinocular Stereo, 3.5X-90X Magnification Four-Zone LED Ring Light

MicroBob
Posts: 1651
Joined: Sun Dec 25, 2016 9:11 am

Re: I thinl I am getting a bit better detail on nematodes.

#2 Post by MicroBob » Wed Jul 31, 2019 12:51 pm

This in absolutely normal. The linear resolution depends on the aperture of the lens. The information content of the image depends on the object size that you see times the resolution. The stronger objectives show a comparatively small image are for the resolution they offer.

10:1 objective n.a 0,25
100:1 objective n.a. 1,25 => linear image size 0,1 x that of the 10:1 objective, resolution 5x more => 0,5x the linear image detail => 0,25x the image detail in the area.

Is it possible that the aperture of your condenser was a little low? Did you oil the condenser?
The stronger the objective the better the slide should be prepared, e.g. thin water film, object close to the underside of the slide. A soon as you have a bigger object in there this will limit the quality.

Bob

Post Reply