Daffodil root tip mitosis

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mrsonchus
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Daffodil root tip mitosis

#1 Post by mrsonchus » Wed Jul 31, 2019 8:09 pm

Hi all, I've been taking a few images of some Daffodil root-tip slides that have a lot of mitosis that I though some may like to have a look at. The files of cells being generated by the root-tip's dividing cells (initials in the root-apical-meristem) are clearly visible also.
Anyway, a few images - chromosomes everywhere!

This is a 10x, 3-image stitch - the cell lineages are quite easily seen,
Image

Chromosomes look to be grouping ('condensing'?) and becoming visible I think,
Image

Image

Image

Chromosomes seperating to opposite ends of cell,
Image

Image

Image

Somewhat random images I'm afraid, I was just practicing with the BX40 and though I'd put some colour up!
John B

Wes
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Re: Daffodil root tip mitosis

#2 Post by Wes » Wed Jul 31, 2019 8:24 pm

Very nice! Did you prepare the sections yourself?

I really like how you can see the mitotic spindle fibers and fragmoplast in the last couple of images. If you can it might be worth checking the spindle under polarization. It has anisotropic properties and interacts with polarized in an interesting manner.

tgss
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Re: Daffodil root tip mitosis

#3 Post by tgss » Wed Jul 31, 2019 9:10 pm

I'm with Wes on these images, John. Excellent images; excellent sections; the mitotic spindles very clearly demonstrated.
Thanks for sharing.
Tom.

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mrsonchus
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Re: Daffodil root tip mitosis

#4 Post by mrsonchus » Wed Jul 31, 2019 9:16 pm

Thanks Wes & Tom - pleased you like them. These are just from this afternoon's 'slide-surfing' during a quiet hour. I've many images from back when I made the slides also. I'll post a few more later tonight.
John B

MichaelG.
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Re: Daffodil root tip mitosis

#5 Post by MichaelG. » Wed Jul 31, 2019 9:24 pm

Predictably excellent work, John
... and the new 'scope is looking good

You make a fine team !!

MichaelG.
Too many 'projects'

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mrsonchus
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Re: Daffodil root tip mitosis

#6 Post by mrsonchus » Wed Jul 31, 2019 9:55 pm

Hi Michael, thanks my friend. I'm really starting to find my way around the BX40 now, and am getting far better with the 60x too! Having a great time with my Botany - many tissues in line for slides once we have just a little cooler weather I think.
John B

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75RR
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Re: Daffodil root tip mitosis

#7 Post by 75RR » Wed Jul 31, 2019 10:00 pm

Very nice detail! Like that you are stitching as well.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
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mrsonchus
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Re: Daffodil root tip mitosis

#8 Post by mrsonchus » Wed Jul 31, 2019 10:03 pm

75RR wrote:Very nice detail! Like that you are stitching as well.
Thanks 75' - yes stitching with the Olympus (and the LED-kit now fitted) is giving me far better equality of background illumination now. No more of those ghastly 'dark corners' hopefully, that ruin many a lower-power objective stitch... Fingers-crossed. :D
John B

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Crater Eddie
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Re: Daffodil root tip mitosis

#9 Post by Crater Eddie » Thu Aug 01, 2019 4:48 pm

Great! Are the higher power images done with the Oly 60x?.
CE
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mrsonchus
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Re: Daffodil root tip mitosis

#10 Post by mrsonchus » Thu Aug 01, 2019 8:42 pm

Yes they are Ed' - I'm just starting to get the hang of the beastie....
A few more images from earlier:

Sieve-plate with 'slime-plug' from Sunflower leaf petiole phloem in TS - I think it was 8 images stacked.
All the rest are single images.
ws_sunflower petiole sieve plate.jpg
ws_sunflower petiole sieve plate.jpg (71.49 KiB) Viewed 3121 times
A few more mitosis images from Daffodil adventure slides,
ws_daffodil root mitosis 2.jpg
ws_daffodil root mitosis 2.jpg (66.25 KiB) Viewed 3121 times
ws_daffodil root mitosis 3.jpg
ws_daffodil root mitosis 3.jpg (67.87 KiB) Viewed 3121 times
ws_daffodil root mitosis.jpg
ws_daffodil root mitosis.jpg (63.03 KiB) Viewed 3121 times
lastly,
ws_daffodil root mitosis 4.jpg
ws_daffodil root mitosis 4.jpg (64.41 KiB) Viewed 3121 times
Last edited by mrsonchus on Thu Aug 01, 2019 10:09 pm, edited 1 time in total.
John B

Hobbyst46
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Re: Daffodil root tip mitosis

#11 Post by Hobbyst46 » Thu Aug 01, 2019 8:50 pm

Excellent images!
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

billbillt
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Re: Daffodil root tip mitosis

#12 Post by billbillt » Thu Aug 01, 2019 9:13 pm

Hi John B.,

Those are incredible quality!..

BillT

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mrsonchus
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Re: Daffodil root tip mitosis

#13 Post by mrsonchus » Thu Aug 01, 2019 10:12 pm

Thanks Bill & Hobby', pleased you like them. :D
John B

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